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Aptamer-based rapid visual biosensing of melamine in whole milk

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Abstract

A rapid and facile visual method for the detection of melamine (MA) in whole milk based on aptamer-modified gold nanoparticles (AuNPs) was described in this paper. This strategy combined aptamer as a MA recognition element with AuNPs as the color indicator, providing a rapid and on-site detection of MA by naked eyes or UV–Vis spectrometer. The whole analytical process could finish in 30 min without any assistance of any instrument. Under the optimized condition, the proposed method could be used to detect MA in whole milk with a detection limit of 1.5 mg/L in naked eyes and 0.5 mg/L with UV–Vis spectrometer. The simple rapid technique provided a promising tool for on-site screening of MA adulterant in milk as well as in-house diagnosis.

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Acknowledgments

We thank Scientific and Research Project of General Administration of Quality Supervision, Inspection Quarantine (AQSIQ) of China (No. 2013QK007), National Natural Science Foundation of China (Grant No. 61307103) and the West Light Foundation of Chinese Academy of Sciences for financial support of this work.

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Inspection Center for Processed Agricultural Products and Condiments, China National Quality Supervision, Chongqing Academy of Metrology and Quality Inspection, Chongqing, 401123, China

    Wen Yun, Hong Li, Shiqi Chen & Dawei Tu

  2. Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China

    Wanyi Xie & Yu Huang

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  1. Wen Yun
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  2. Hong Li
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  3. Shiqi Chen
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  4. Dawei Tu
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  5. Wanyi Xie
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  6. Yu Huang
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Correspondence to Yu Huang.

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Yun, W., Li, H., Chen, S. et al. Aptamer-based rapid visual biosensing of melamine in whole milk. Eur Food Res Technol 238, 989–995 (2014). https://doi.org/10.1007/s00217-014-2166-3

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  • DOI: https://doi.org/10.1007/s00217-014-2166-3

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