Skip to main content
SpringerLink
Log in

Analysis of methanol–ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

A portable bienzymatic analytical system was developed for the chronoamperometric analysis of methanol–ethanol mixtures. The system consists of two biosensors, one based on alcohol dehydrogenase (ADH) that responds only to the ethanol and the second one based on alcohol oxidase (AOX) that responds to both methanol and ethanol. The transducers were screen-printed electrodes (SPEs) modified with mediators: Meldola blue for ADH and Co-phthalocyanine for AOX. The calibration graph of the ADH biosensor is linear between 0.3 and 8 mmol/L ethanol. The AOX biosensor is able to quantify both analytes in mixtures that contain methanol between 3 and 70 mmol/L and ethanol ranging from 15 to 110 mmol/L. Interferences due to non-specific oxidations from common oxidizable compounds like gallic acid and ascorbic acid were smaller in the case of transducer based on Meldola blue. The analytical system was successfully tested on real samples: non-alcoholised beer (NAB) spiked with ethanol or methanol and a falsified rose wine (FRW).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

ADH:

Alcohol dehydrogenase

AOX:

Alcohol oxidase

SPEs:

Screen-printed electrodes

WE:

Working electrode

NAD+/NADH:

Nicotinamide adenine dinucleotide oxidized/reduced forms

FAD:

Flavin adenine dinucleotide

K M :

Michaelis constant

PVA-SbQ:

Polyvinyl alcohol containing stilbazolium groups

NAB:

Non-alcoholic beer

FRW:

Falsified rose wine

References

  1. van Wyk JPH (2001) Trends Biotech 19:172–177. doi:10.1016/S0167-7799(01)01601-8

    Article  Google Scholar 

  2. Jacobs P, Dewé W, Flament A, Gibella M, Ceccato A (2006) J Pharm Biomed Anal 40(2):294–304. doi:10.1016/j.jpba.2005.06.036

    Article  CAS  Google Scholar 

  3. Klein H, Leubolt R (1993) J Chromatogr A 640(1–2):259–270. doi:10.1016/0021-9673(93)80189-F

    Article  CAS  Google Scholar 

  4. Harmon BJ, Patterson DH, Regnier FE (1993) J Chromatogr A 657(2):429–434. doi:10.1016/0021-9673(93)80298-M

    Article  CAS  Google Scholar 

  5. Azmak D (2006) Leg Med 8(1):39–42. doi:10.1016/j.legalmed.2005.07.002

    Article  CAS  Google Scholar 

  6. Azevedo AM, Prazeres DMF, Cabral JMS, Fonseca LP (2005) Biosens Bioelectr 21:235–247. doi:10.1016/j.bios.2004.09.030

    Article  CAS  Google Scholar 

  7. Sekine Y, Suzuki M, Takeuchi T, Tamiya E, Karube I (1993) Anal Chim Acta 280:179–184. doi:10.1016/0003-2670(93)85119-5

    Article  CAS  Google Scholar 

  8. de Prada GV, Pena N, Mena ML, Reviejo AJ, Pingarron JM (2003) Biosens Bioelectr 18:1279–1288. doi:10.1016/S0956-5663(03)00074-5

    Article  CAS  Google Scholar 

  9. Gulce H, Gulce A, Kavanoz M, Coskun H, Yildiz A (2002) Biosens Bioelectr 17:517–521. doi:10.1016/S0956-5663(02)00008-8

    Article  CAS  Google Scholar 

  10. Avramescu A, Noguer T, Magearu V, Marty JL (2001) Anal Chim Acta 433:81–88. doi:10.1016/S0003-2670(01)00823-6

    Article  CAS  Google Scholar 

  11. Bucur B, Mallat E, Gurban AM, Gocheva Y, Velasco C, Marty JL, Noguer T (2006) Biosens Bioelectr 21(12):2290–2297. doi:10.1016/j.bios.2005.10.022

    Article  CAS  Google Scholar 

  12. Andreescu S, Barthelmebs L, Marty JL (2002) Anal Chim Acta 464:171–180. doi:10.1016/S0003-2670(02)00518-4

    Article  CAS  Google Scholar 

  13. Chaubey A, Malhotra BD (2002) Biosens Bioelectr 17:441–456. doi:10.1016/S0956-5663(01)00313-X

    Article  CAS  Google Scholar 

  14. Gorton L, Dominguez E (2002) Rev Mol Biotechnol 82:371–392. doi:10.1016/S1389-0352(01)00053-8

    Article  CAS  Google Scholar 

  15. Jordan J, Ciolkosz MK (1991) J Solut Chem 20(10):995–1000. doi: 10.1007/BF00663998

    Article  CAS  Google Scholar 

  16. Vasilescu A, Noguer T, Andreescu S, Calas-Blanchard C, Bala C, Marty JL (2003) Talanta 59:751–765. doi:10.1016/S0039-9140(02)00614-8

    Article  CAS  Google Scholar 

  17. Abass AK, Hart JP, Cowell DC, Chappell A (1998) Anal Chim Acta 373(1):1–8. doi:10.1016/S0003-2670(98)00368-7

    Article  CAS  Google Scholar 

  18. Curulli A, Valentini F, Orlanduci S, Terranova ML, Palleschi G (2004) Biosens Bioelectr 20:1223–1232. doi:10.1016/j.bios.2004.06.026

    Article  CAS  Google Scholar 

  19. Gilmartin MAT, Hart JP, Birch BJ (1994) Analyst 119:243–252. doi: 10.1039/AN9941900243

    Article  CAS  Google Scholar 

  20. Gilmartin MAT, Hart JP (1994) Analyst 119:2331–2336. doi: 10.1039/AN9941902331

    Article  CAS  Google Scholar 

  21. Leca B, Marty JL (1997) Biosens Bioelectr 12:1083–1088. doi:10.1016/S0956-5663(97)00075-4

    Article  CAS  Google Scholar 

  22. Lushine KA, Harris CR, Holger JS (2003) J Emerg Med 24(4):433–436. doi:10.1016/S0736-4679(03)00041-6

    Article  Google Scholar 

Download references

Acknowledgements

Bucur Bogdan is a post-doctoral fellow of Romanian Ministry of Education and Research through Grant PD-011/2005. The work was supported by Romanian Ministry of Education and Research through Grant CEEX-156/2006. The Meldola blue modified screen-printed paste was a gift from Gwent Electronic Materials (UK). The authors wish to thank Prof. Jean-Louis Marty (BIOMEM-University of Perpignan, France) for the SPEs and PVA polymer and Prof. Didier Fournier (Paul Sabatier University, Toulouse, France) for helpful discussions on enzymatic kinetic.

Author information

Authors and Affiliations

  1. Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1-7, Polizu, Bucharest, 011061, Romania

    Bogdan Bucur

  2. Centre of Bioanalysis, National Institute of Biological Sciences, 296, Splaiul Independentei, Bucharest, 060031, Romania

    Gabriel Lucian Radu

  3. Scientifical Research Center for Defence NBC and Ecology, Sos. Oltenitei, nr.225, Sector 4, Bucharest, Romania

    Constantin Nicolae Toader

Authors
  1. Bogdan Bucur
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gabriel Lucian Radu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Constantin Nicolae Toader
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bogdan Bucur.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bucur, B., Radu, G.L. & Toader, C.N. Analysis of methanol–ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase. Eur Food Res Technol 226, 1335–1342 (2008). https://doi.org/10.1007/s00217-007-0662-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-007-0662-4

Keywords

Search

Navigation