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Strategies for the detection of target analytes using microfluidic paper-based analytical devices

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Abstract

Microfluidic paper-based analytical devices (μPADs) have developed rapidly in recent years, because of their advantages, such as small sample volume, rapid detection rates, low cost, and portability. Due to these characteristics, they can be used for in vitro diagnostics in the laboratory, or in the field, for a variety of applications, including food evaluation, disease screening, environmental monitoring, and drug testing. This review will present various detection methods employed by μPADs and their respective applications for the detection of target analytes. These include colorimetry, electrochemistry, chemiluminescence (CL), electrochemiluminescence (ECL), and fluorescence-based methodologies. At the same time, the choice of labeling material and the design of microfluidic channels are also important for detection results. The construction of novel nanocomponents and different smart structures of paper-based devices have improved the performance of μPADs and we will also highlight some of these in this manuscript. Additionally, some key challenges and future prospects for the use of μPADs are briefly discussed.

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Funding

We are grateful for the financial support by the National Key Research and Development Program of China (Grant Nos. 2017FYA0205303 and 2017FYA0205301), the National Natural Science Foundation of China (Grant No. 81672247), Shanghai Science and Technology Fund (No.15DZ225200), and Funding of SJTU (No ZH2018QNA03 and YG2019QNB09).

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Authors and Affiliations

  1. Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai Engineering Research Center for Intelligent Diagnosis and Treatment Instruments, Key Laboratory of Thin Film and Microfabrication (Ministry of Education), Shanghai, 200240, China

    Wei Zheng, Kan Wang, Chujun Zheng, Bo Cao, Qi Qin & Daxiang Cui

  2. State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China

    Qinghui Jin

  3. Faculty of Electrical Engineering and Computer Science, Ningbo University, Ningbo, Zhejiang, 315211, China

    Qinghui Jin

  4. School of Naval Architecture, Ocean & Civil Engineering, Shanghai Jiao Tong University, Shanghai, 200240, China

    Hao Xu

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  1. Wei Zheng
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  2. Kan Wang
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Correspondence to Kan Wang.

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Zheng, W., Wang, K., Xu, H. et al. Strategies for the detection of target analytes using microfluidic paper-based analytical devices. Anal Bioanal Chem 413, 2429–2445 (2021). https://doi.org/10.1007/s00216-021-03213-x

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