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A time-resolved luminescence aptasensor of ofloxacin based on rolling circle amplification and magnetic separation

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Abstract

A novel sensitive, competitive, and time-resolved luminescence sensor for the detection of ofloxacin (OFL) was developed in this study. The sensor used OFL-specific aptamer as a recognition molecule and rolling circle amplification (RCA) as a signal amplification tool. In this way, the time-resolved luminescence can not only avoid background noise from sample, but also provide robust luminescence for detection. Besides, the separation and enrichment of target veterinary drug can be conducted assisted by magnetic treatment. Under optimal conditions, the logarithmic correlation between the concentration of OFL and the luminescence intensity was found to be linear in the range of 5 × 10−11–5 × 10−8 mol L−1 (R2 = 0.9988), with a detection limit (LOD) of 32.1 pmol L−1. Furthermore, this method was applied to the determination of OFL in chicken and pork samples, exhibiting good recovery (72.5–100%) and repeatability (RSD < 10.0%). These results confirm that this novel established method has good application potential for the detection of OFL in food samples.

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Acknowledgments

This work was partly supported by the National Natural Science Foundation of China (31801647), “Chunhui Program” Cooperation Scientific Research of Ministry of Education of China (Z2016135), the Sichuan Science and Technology Program (2018JY0194, 2018SZ0340), the “Xihua Cup” Students Innovation and Entrepreneurship Project of Xihua University (2019082), and the Young Scholars Reserve Talent Program of Xihua University.

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Authors and Affiliations

  1. School of Food and Biological Engineering, Xihua University, Chengdu, 610039, China

    Yukun Huang, Chong Wang, Qiuyu Huo, Yaning Song, Lijun Wang, Xiao Yang & Xianggui Chen

  2. Key Laboratory of Food Non-Thermal Processing, Engineering Technology Research Center of Food Non-Thermal Processing, Yibin Xihua University Research Institute, Yibin, 644004, China

    Yukun Huang & Xianggui Chen

  3. Sichuan Institute for Food and Drug Control, Chengdu, 611731, China

    Gang Du

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  1. Yukun Huang
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Correspondence to Yukun Huang or Xianggui Chen.

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Huang, Y., Wang, C., Huo, Q. et al. A time-resolved luminescence aptasensor of ofloxacin based on rolling circle amplification and magnetic separation. Anal Bioanal Chem 412, 4555–4563 (2020). https://doi.org/10.1007/s00216-020-02708-3

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  • DOI: https://doi.org/10.1007/s00216-020-02708-3

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