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Colorimetric determination of glutathione in human serum and cell lines by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite

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Abstract

In this study, we developed a simple colorimetric approach to detect glutathione (GSH). The proposed approach is based on the ability of CuS-PDA-Au composite material to catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to ox-TMB to induce a blue color with an absorption peak centered at 652 nm. However, the introduction of GSH can result in a decrease in oxidized TMB; similarly, it can combine with Au nanoparticles (Au NPs) on the surface of CuS-PDA-Au composite material. Both approaches can result in a fading blue color and a reduction of the absorbance at 652 nm. Based on this above, we proposed a technique to detect GSH quantitatively and qualitatively through UV-Vis spectroscopy and naked eye, respectively. This approach demonstrates a low detection limit of 0.42 μM with a broad detection range of 5 × 10−7–1 × 10−4 M with the assistance of UV-Vis spectroscopy. More importantly, this approach is convenient and rapid. This method was successfully applied to GSH detection in human serum and cell lines.

A colorimetric approach has been developed by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite for sensitive glutathione detection.

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Acknowledgments

We thank the anonymous reviewers for their valuable suggestions.

Funding

This work was supported by a grant from the National Natural Science Foundation of China (21305097) and Two-way Support Plan Foundation of Sichuan Agricultural University.

Author information

Author notes

  1. Yanying Wang and Yaqin Liu contributed equally to this work.

Authors and Affiliations

  1. College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya’an, 625014, Sichuan, China

    Yanying Wang, Yaqin Liu, Xiaoyan Zhu, Li Yang, Ping Zou, Hanbing Rao & Xianxiang Wang

  2. Suzhou Institute of Systems Medicine, Suzhou, 215123, Jiangsu, China

    Fang Ding

  3. Center for Systems Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100005, China

    Fang Ding

  4. Key Laboratory of Polar Materials and Devices, Ministry of Education, Department of Electronic Engineering, East China Normal University, Shanghai, 200241, China

    Qingbiao Zhao

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  1. Yanying Wang
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  2. Yaqin Liu
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  3. Fang Ding
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  4. Xiaoyan Zhu
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Corresponding authors

Correspondence to Hanbing Rao or Xianxiang Wang.

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The project was approved by the Third People’s Hospital of Chengdu. All experiments were performed in compliance with the relevant laws and institutional guidelines.

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Wang, Y., Liu, Y., Ding, F. et al. Colorimetric determination of glutathione in human serum and cell lines by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite. Anal Bioanal Chem 410, 4805–4813 (2018). https://doi.org/10.1007/s00216-018-1117-4

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