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Thiophene-based rhodamine as selectivef luorescence probe for Fe(III) and Al(III) in living cells

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Abstract

The thiophene-modified rhodamine 6G (GYJ) has been synthesized as a novel chemosensor. The sensor has sufficiently high selectivity and sensitivity for the detection of Fe3+ and Al3+ ions (M3+) by fluorescence and ultraviolet spectroscopy with a strong ability for anti-interference performance. The binding ratio of M3+-GYJ complex was determined to be 2:1 according to the Job’s plot. The binding constants for Fe3+ and Al3+ were calculated to be 3.91 × 108 and 5.26 × 108 M−2, respectively. All these unique features made it particularly favorable for cellular imaging applications. The obvious fluorescence microscopy experiments demonstrated that the probes could contribute to the detection of Fe3+ and Al3+ in related cells and biological organs with satisfying resolution.

GYJ has high selectivity and sensitivity for the detection of Fe(III) and Al(III) with the binding ratio of 2:1.

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Acknowledgements

We are grateful to the National Natural Science Foundation of China (21172127) for the financial support.

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Authors and Affiliations

  1. Key Laboratory of Eco-chemical Engineering, Ministry of Education, Qingdao University of Science and Technology, Qingdao, 266042, China

    Kun-Peng Wang, Ju-Peng Chen, Si-Jie Zhang, Yang Lei, Shaojin Chen, Xin-Hong Zhou & Zhi-Qiang Hu

  2. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Hua Zhong

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  1. Kun-Peng Wang
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Correspondence to Zhi-Qiang Hu.

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Clarification for source of living cells

The HeLa cells were obtained from Prof. Hai-Yu Hu’s Lab (Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China).

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Wang, KP., Chen, JP., Zhang, SJ. et al. Thiophene-based rhodamine as selectivef luorescence probe for Fe(III) and Al(III) in living cells. Anal Bioanal Chem 409, 5547–5554 (2017). https://doi.org/10.1007/s00216-017-0490-8

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  • DOI: https://doi.org/10.1007/s00216-017-0490-8

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