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Microscope-assisted UV-initiated preparation of well-defined porous polymer monolithic plugs in glass microchips for peptide preconcentration

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Abstract

Herein, highly defined monolithic beds were prepared in glass microchips by photopolymerization of ethylene glycol methacrylate phosphate (EGMP), acrylamide, and N,N′-methylenebisacrylamide (BAA) using an epifluorescence microscope as UV-irradiation source. Such a fast and easy method allowed precise control of (i) the edge shape, (ii) the location along the microchannel, and (iii) the length of the monolithic plugs within glass microchips. The addition of hydroquinone, a polymerization inhibitor, to the prepolymerization mixture was beneficial for achieving local and robust incorporation of monoliths with sharp edges within microchannels. The monolith length was easily tuned from 160 to 400 μm through simple change in the magnification of the objective and was found to be repeatable (relative standard deviation <7.5%). Further application for on-chip monolith-assisted solid - phase extraction is demonstrated for fluorescently labeled peptide. Both binding and subsequent elution behaviors were found to fully agree with a cation-exchange mechanism in concordance with the presence of phosphate groups at the monolith surface.

In-chip microscope-UV-synthesis of monolithic plugs with sharp edges

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Acknowledgements

The authors wish to thank the Natural Sciences and Engineering Research Council of Canada (NSERC) for the financial support and the DIM Analytics of France for the post-doctoral scholarship.

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    Authors and Affiliations

    1. Univ. Paris-Est, ICMPE (UMR7182), CNRS, UPEC, 2 Rue Henri Dunant, 94320, Thiais, France

      Szymon Dziomba, Mohamed Guerrouache & Benjamin Carbonnier

    2. Institut Galien Paris Sud, UMR8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290, Châtenay-Malabry, France

      Szymon Dziomba, Monica Araya-Farias, Myriam Taverna & N. Thuy Tran

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    1. Szymon Dziomba
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    2. Monica Araya-Farias
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    3. Myriam Taverna
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    4. Mohamed Guerrouache
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    5. Benjamin Carbonnier
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    6. N. Thuy Tran
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    Correspondence to N. Thuy Tran.

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    Szymon Dziomba and Monica Araya-Farias contributed equally to this work.

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    Dziomba, S., Araya-Farias, M., Taverna, M. et al. Microscope-assisted UV-initiated preparation of well-defined porous polymer monolithic plugs in glass microchips for peptide preconcentration. Anal Bioanal Chem 409, 2155–2162 (2017). https://doi.org/10.1007/s00216-016-0161-1

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    • DOI: https://doi.org/10.1007/s00216-016-0161-1

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