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A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing

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Abstract

For the first time, a multi-analyte biosensor platform has been developed using the label-free 1-lambda-reflectometry technique. This platform is the first, which does not use imaging techniques, but is able to perform multi-analyte measurements. It is designed to be portable and cost-effective and therefore allows for point-of-need testing or on-site field-testing with possible applications in diagnostics. This work highlights the application possibilities of this platform in the field of animal testing, but is also relevant and transferable to human diagnostics. The performance of the platform has been evaluated using relevant reference systems like biomarker (C-reactive protein) and serology (anti-Salmonella antibodies) as well as a panel of real samples (animal sera). The comparison of the working range and limit of detection shows no loss of performance transferring the separate assays to the multi-analyte setup. Moreover, the new multi-analyte platform allows for discrimination between sera of animals infected with different Salmonella subtypes.

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Acknowledgments

This work was funded by the Bundesministerium für Wirtschaft und Technologie (BMWi) and the Allianz Industrie und Forschung (AIF) within the project “Optische Tierdiagnostik”, IGF- Nr. 16061 N/1. Special thanks go to Klaus Haberstroh (Qiagen Lake Constance GmbH, Germany) for providing the ESE-LOG USB.

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Authors and Affiliations

  1. Institute of Physical and Theoretical Chemistry (IPTC), Eberhard Karls Universität Tübingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany

    Melanie Ewald & Günter Gauglitz

  2. Biametrics GmbH, Auf der Morgenstelle 18, 72076, Tuebingen, Germany

    Peter Fechner

Authors
  1. Melanie Ewald
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  2. Peter Fechner
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  3. Günter Gauglitz
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Correspondence to Günter Gauglitz.

Additional information

Published in the topical collection Direct Optical Detection with guest editors Guenter Gauglitz and Jiri Homola.

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Ewald, M., Fechner, P. & Gauglitz, G. A multi-analyte biosensor for the simultaneous label-free detection of pathogens and biomarkers in point-of-need animal testing. Anal Bioanal Chem 407, 4005–4013 (2015). https://doi.org/10.1007/s00216-015-8562-0

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  • DOI: https://doi.org/10.1007/s00216-015-8562-0

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