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Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

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Abstract

Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

The quantitative results of Gd@C82(OH)22 in single cells determined by SC-ICP-MS and acid digestion method, respectively

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Acknowledgments

This work was supported by the National Basic Research Program (973 Program: 2011CB933403) and the National Natural Science Foundation of China (21175136, 11275214, and 11375211).

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Authors and Affiliations

  1. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China

    Ling-Na Zheng, Meng Wang, Lei-Chao Zhao, Bao-Yun Sun, Bing Wang, Han-Qing Chen, Yu-Liang Zhao, Zhi-Fang Chai & Wei-Yue Feng

  2. School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, China

    Lei-Chao Zhao

  3. CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology of China, Chinese Academy of Sciences, Beijing, 100190, China

    Yu-Liang Zhao

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  1. Ling-Na Zheng
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Correspondence to Wei-Yue Feng.

Additional information

Published in the topical collection Spectrochemical Plasmas for Clinical and Biochemical Analysis with guest editors Alfredo Sanz-Medel and María Montes Bayón.

Ling-Na Zheng and Meng Wang contributed equally to this work.

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Zheng, LN., Wang, M., Zhao, LC. et al. Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry. Anal Bioanal Chem 407, 2383–2391 (2015). https://doi.org/10.1007/s00216-014-8422-3

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