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Reproducibility of retention time and peak area in comprehensive two-dimensional liquid chromatography

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Abstract

Comprehensive two-dimensional liquid chromatography is used to separate anionic, nonionic, and amphoteric surfactants by substance class, alkyl chain distribution, and degree of ethoxylation. A nearly orthogonal system with a hydrophilic interaction chromatography (HILIC) phase in the first and a reversed-phase material in the second dimension is applied to generate a separation with maximum peak capacity. The potential of the developed method is demonstrated by the reproducibility of retention time and peak area, which shows standard deviations less than 5 % and the analysis of real samples. An external calibration and the standard addition method were applied to determine unknown concentrations for the alkyl chain homologues of a betaine and for one ethoxylate (EO) homologue of a fatty alcohol ethoxylate in a sample mixture.

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Acknowledgments

Financial support is gratefully acknowledged to BASF Personal Care and Nutrition GmbH (Duesseldorf, Germany) for the funding and the chemical products and to Dr. Maisch GmbH (Ammerbuch-Entringen, Germany) for providing the reversed-phase columns. We thank Agilent (Waldbronn, Germany) for the test version of the new LCxLC Add-On for Chemstation Version B.04.03 and an additional UHPLC pump.

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Authors and Affiliations

  1. Institute for Pure and Applied Mass Spectrometry, University of Wuppertal, Gaussstr. 20, 42119, Wuppertal, Germany

    Victoria Elsner & Oliver J. Schmitz

  2. BASF Personal Care and Nutrition GmbH, Henkelstraße 67, 40589, Düsseldorf, Germany

    Volker Wulf & Michaela Wirtz

  3. Applied Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5-7, 45141, Essen, Germany

    Oliver J. Schmitz

Authors
  1. Victoria Elsner
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  2. Volker Wulf
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  3. Michaela Wirtz
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  4. Oliver J. Schmitz
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Corresponding author

Correspondence to Oliver J. Schmitz.

Additional information

Published in the topical collection Multidimensional Chromatography with guest editors Torsten C. Schmidt, Oliver J. Schmitz, and Thorsten Teutenberg.

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Elsner, V., Wulf, V., Wirtz, M. et al. Reproducibility of retention time and peak area in comprehensive two-dimensional liquid chromatography. Anal Bioanal Chem 407, 279–284 (2015). https://doi.org/10.1007/s00216-014-8090-3

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  • DOI: https://doi.org/10.1007/s00216-014-8090-3

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