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Use of gel permeation chromatography for clean-up in the analysis of coccidiostats in eggs by liquid chromatography–tandem mass spectrometry

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Abstract

An analytical method for determination and confirmation of nine coccidiostatics in eggs is reported. Ethyl acetate is used as extraction solvent, with satisfactory results, and simple automated clean-up is based on gel-permeation chromatography (GPC) . The target compounds are then analysed by liquid chromatography–electrospray ionization–tandem mass spectrometry. The method was validated in-house in accordance with Commission Decision 2002/657/EC. Trueness and precision were determined at four concentrations, and the mean errors obtained were <10 %, with relative standard deviations ranging from 3 to 18 %. For three non-authorized coccidiostatics (clopidol, ethopabate, and ronizadole), decision limit and detection capability were in the ranges 0.12–0.16 and 0.18–0.23 μg kg−1, respectively. The results obtained prove the suitability of this new analytical method for routine monitoring of these substances in eggs.

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Acknowledgments

Financial support from the Spanish Ministerio de Ciencia y Tecnología (CiCYT Project AGL2008-05578-C05-03) is gratefully acknowledged.

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Authors and Affiliations

  1. Departament de Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franquès 1-11, 08028, Barcelona, Spain

    J. Chico, R. Companyó, M. D. Prat & M. Granados

  2. Servei de Química, Àrea Anàlisi Orgànica, Laboratori Agència de Salut Pública de Barcelona, Av. Drassanes 13, 08001, Barcelona, Spain

    A. Rúbies & F. Centrich

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  1. J. Chico
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  2. A. Rúbies
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  3. F. Centrich
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  5. M. D. Prat
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  6. M. Granados
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Correspondence to M. Granados.

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Chico, J., Rúbies, A., Centrich, F. et al. Use of gel permeation chromatography for clean-up in the analysis of coccidiostats in eggs by liquid chromatography–tandem mass spectrometry. Anal Bioanal Chem 405, 4777–4786 (2013). https://doi.org/10.1007/s00216-013-6896-z

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  • DOI: https://doi.org/10.1007/s00216-013-6896-z

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