Abstract
The molecularly imprinted polymeric microspheres (MIPMs, 3∼5 μm), used as high-performance liquid chromatography (HPLC) and solid-phase extraction (SPE) packing materials for anti-AIDS drug emtricitabine (FTC), were synthesized by precipitation polymerization. The effects of ratio of chloroform to acetonitrile on the morphology and diameter of MIPMs were investigated. The prepared MIPMs were characterized by HPLC. The imprinting factor (2.26) suggests that the resultant MIPMs exhibit good recognition and affinity to FTC. In addition, the MIPMs were used in SPE as packing material for separation and enrichment of FTC. The recovery of FTC on MIPMs cartridge was 97.6 % in standard solution. Finally, the MIPMs cartridge was applied to extract the FTC in human serum samples. Impurities in sample have been mostly removed, and the average recovery of 92.5 % was obtained with a detection limit of 0.005 μg/mL and a linear range of 0.02∼4.0 μg/mL. The method established can be used to monitor the FTC in human serum sample with good accuracy and selectivity.
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References
Rockstroh JK, Lennox JL, DeJesus E, Saag MS, Lazzarin A, Wan H, Walker ML, Xu X, Zhao J, Teppler H, DiNubile MJ, Rodgers AJ, Nguyen BY, Leavitt R, Sklar P (2011) Clin Infect Dis 53:807–816
Venhoff N, Setzer B, MelkaoUi K, Walker UA (2007) Antivir Ther 12:1075–1085
DeJesus E, Ruane P, McDonald C, Garcia F, Sharma S, Corales R, Ravishankar J, Khanlou H, Shamblaw D, Ecker J, Ebrahimi R, Flaherty J (2008) HIV Clin Tri 9:103–114
Pendela M, Mamade DA, Hoogmartens J, Van Schepdael A, Adams E (2010) Talanta 82:125–128
Jansen RS, Rosing H, Kromdijk W, ter Heine R, Schellens JHM, Beijnen JH (2010) J Chromatogr B 878:621–627
Nirogi R, Bhyrapuneni G, Kandikere V, Mudigonda K, Komarneni P, Aleti R, Mukkanti K (2009) Biomed Chromatogr 23:371–381
Gomes NA, Vaidya VV, Pudage A, Joshi SS, Parekh SA (2008) J Pharm Biomed Anal 48:918–926
Ashenafi D, Verbeek A, Hoogmartens J, Adams E (2009) J Separ Sci 32:1823–1830
Seshachalam U, Haribabu B, Chandrasekhar KB (2007) J Separ Sci 30:999–1004
Lai JP, Wang J, Cai ZW (2008) J Chromatogr B 868:1–12
Rezk NL, Crutchley RD, Kashuba ADM (2005) J Chromatogr B 822:201–208
Lai JP, Niessner R, Knopp D (2004) Anal Chim Acta 522:137–144
Lai JP, He XW, Chen F (2003) Anal Bioanal Chem 377:208–213
Turiel E, Martin-Esteban A (2004) Anal Bioanal Chem 378:1876–1886
Gu JY, Zhang H, Yuan G, Chen LR, Xu XJ (2011) J Chromatogr A 1218:8150–8155
Wang CY, Javadi A, Ghaffari M, Gong SQ (2010) Biomater 31:4944–4951
Yang T, Li YH, Wei S, Li Y, Deng AP (2008) Anal Bioanal Chem 391:2905–2914
Guo L, Deng QL, Fang GZ, Gao W, Shuo W (2011) J Chromatogr A 1218:6271–6277
Wang XH, Chen LR, Xu XJ (2011) Anal Bioanal Chem 401:1423–1432
Qiao FX, Geng YR, He CQ, Wu YP, Pan PY (2011) J Chromatogr B 879:2891–2896
Ambrosini S, Shinde S, De Lorenzi E, Sellergren B (2012) Analyst 137:249–254
Ameli A, Alizadeh N (2011) Anal Chim Acta 707:62–68
Duy SV, Lefebvre-Tournier I, Pichon V, Hugon-Chapuis F, Puy JY, Perigaud C (2009) J Chromatogr B 877:1101–1108
Lai JP, Yang ML, Niessner R, Knopp D (2007) Anal Bioanal Chem 389:405–412
Dai CM, Zhou XF, Zhang YL, Liu SG, Zhang J (2011) J Hazard Mater 198:175–181
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This study is financially supported by the National Nature Science Foundation of China (No: 21107018).
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Lai, Jp., Xie, L., Sun, H. et al. Synthesis and evaluation of molecularly imprinted polymeric microspheres for highly selective extraction of an anti-AIDS drug emtricitabine. Anal Bioanal Chem 405, 4269–4275 (2013). https://doi.org/10.1007/s00216-013-6818-0
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DOI: https://doi.org/10.1007/s00216-013-6818-0