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Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography–tandem mass spectrometry

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Abstract

A method using microextraction by packed sorbent (MEPS) and gas chromatography–tandem mass spectrometry (GC-MS/MS) is described for the determination of seven antipsychotic drugs in human plasma. The studied compounds were chlorpromazine (CPZ), haloperidol (HAL), cyamemazine, quetiapine, clozapine, olanzapine (OLZ), and levomepromazine; promazine, protriptyline, and deuterated CPZ were used as internal standards. The validation parameters included selectivity, linearity and limits of detection and quantitation, intra- and interday precision and trueness, recovery, and stability and were studied according to internationally accepted guidelines. The method was found to be linear between the lower limit of quantitation and 1000 ng/mL, except for OLZ and HAL (200 ng/mL), with determination coefficients higher than 0.99 for all analytes, and extraction efficiencies ranged from 62 to 92 %. Intra- and interday precision ranged from 0.24 to 10.67 %, while trueness was within a ±15 % interval from the nominal concentration for all analytes at all studied levels. MEPS has shown to be a rapid procedure for the determination of the selected antipsychotic drugs in human plasma, allowing reducing the handling time and the costs of analysis. Furthermore, GC-MS/MS has demonstrated to be a powerful tool for the simultaneous quantitation of the studied compounds, enabling obtaining adequate selectivity and sensitivity using a sample volume of as low as 0.25 mL.

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Acknowledgments

The authors acknowledge the Protocol UBI/Santander-Totta in the form of two fellowships (Ivo Moreno and Beatriz da Fonseca), the program COMPETE and the Portuguese Foundation for Science and Technology (PEst-C/SAU/UI0709/2011).

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Authors and Affiliations

  1. CICS-UBI-Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6201-556, Covilhã, Portugal

    B. M. da Fonseca, I. E. D. Moreno, J. A. Queiroz & E. Gallardo

  2. Instituto Nacional de Medicina Legal-Delegação do Sul, Rua Manuel Bento de Sousa, 3, 1169-219, Lisboa, Portugal

    M. Barroso & S. Costa

Authors
  1. B. M. da Fonseca
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  2. I. E. D. Moreno
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  3. M. Barroso
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  4. S. Costa
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  5. J. A. Queiroz
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  6. E. Gallardo
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Corresponding author

Correspondence to M. Barroso.

Additional information

Published in the special paper collection Forensic Toxicology with guest editors Kazuhito Watanabe and Satoshi Chinaka.

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da Fonseca, B.M., Moreno, I.E.D., Barroso, M. et al. Determination of seven selected antipsychotic drugs in human plasma using microextraction in packed sorbent and gas chromatography–tandem mass spectrometry. Anal Bioanal Chem 405, 3953–3963 (2013). https://doi.org/10.1007/s00216-012-6695-y

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  • DOI: https://doi.org/10.1007/s00216-012-6695-y

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