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Development of antibody-labelled superparamagnetic nanoparticles for the visualisation of benzo[a]pyrene in porous media with magnetic resonance imaging

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Abstract

Biogeochemical interfaces in soil are dynamic in the spatial and temporal domain and require advanced visualisation and quantification tools to link in vitro experiments with natural systems. This study presents the development, characterization and application of functional nanoparticles coated with monoclonal antibodies to visualise the distribution of benzo[a]pyrene in porous media using magnetic resonance imaging. The labelled particles are 450 nm in diameter and interact with benzo[a]pyrene covalently bound to silanized silica gel. They did not bind to benzo[a]pyrene adsorbed to plain silica gel. Although unspecific filtration was low, washing steps are required for visualisation. The ability to visualise benzo[a]pyrene is inversely correlated to the heterogeneity of the soil materials. There are access restrictions to narrow pore spaces which allow the visualisation of only those pathways which are also accessible to bacteria and hydrocolloids. The production of the particles is applicable to other antibodies which extends the range of potential target contaminants.

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Acknowledgements

This project was funded by the DFG (Ba 1592/5-1) in the framework of the priority program “Biogeochemical Interfaces in Soil” (SPP 1315). NMR relaxometry measurements were funded by the DFG (SCHA 849/8-2). We gratefully acknowledge support from Dr. K. Achterhold (TUM, Mössbauer spectroscopy), Prof. Dr. T. F. Fässler (TUM, SQUID), M. Hanzlik (TUM, TEM), Dr. N. P. Ivleva (TUM, Raman spectroscopy) and Dr. A. Wunderlich (Univ. Ulm, MRI).

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Authors and Affiliations

  1. Institute of Hydrochemistry, TUM, Munich, 81377, Germany

    Martin Rieger, Reinhard Niessner, Michael Seidel & Thomas Baumann

  2. Institute of Environmental Sciences, University Koblenz-Landau, 76829, Landau, Germany

    Gabriele E. Schaumann & Yamuna Kunhi Mouvenchery

Authors
  1. Martin Rieger
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  2. Gabriele E. Schaumann
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  3. Yamuna Kunhi Mouvenchery
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  4. Reinhard Niessner
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  5. Michael Seidel
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  6. Thomas Baumann
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Correspondence to Thomas Baumann.

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Published in the topical collection Analytical Challenges in Environmental and Geosciences with guest editor Christian Zwiener.

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Rieger, M., Schaumann, G.E., Mouvenchery, Y.K. et al. Development of antibody-labelled superparamagnetic nanoparticles for the visualisation of benzo[a]pyrene in porous media with magnetic resonance imaging. Anal Bioanal Chem 403, 2529–2540 (2012). https://doi.org/10.1007/s00216-012-6044-1

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  • DOI: https://doi.org/10.1007/s00216-012-6044-1

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