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Bioaccessibility of total arsenic and arsenic species in seafood as determined by a continuous online leaching method

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Abstract

A continuous leaching method coupled online with inductively coupled plasma mass spectrometry (ICP-MS) detection was used to assess the maximum bioaccessibility of arsenic (As) in seafood samples. The method simulates continuous-flow digestion by successively pumping artificial saliva, gastric and intestinal juices through a mini-column of powdered sample directly connected to the nebuliser of an ICP-MS instrument. The method allows the real-time measurement of As being released by a given reagent. Because the analyte is continuously removed from the system, in contrast to batch methods, the dissolution equilibrium is driven to the right, hence quickly providing information about the worst-case scenario. Following consecutive leaching by the digestive reagents, the leachates were subject to speciation analysis by ion-exchange chromatography with ICP-MS detection to determine the arsenic species released. Finally, the remaining residue from the mini-column was fully digested to verify mass balance. The method was used to determine the bioaccessibility of total As and As species in four certified reference materials and in several real seafood samples. The mass balance was verified in each case. Generally speaking, the non-toxic form was easily released whereas the inorganic forms were poorly bioaccessible.

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Acknowledgment

The authors wish to thank J.C. Leblanc (ANSES DER) for allowing us to use TDS2 and Calipso samples.

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Authors and Affiliations

  1. Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail, Laboratoire de Sécurité des Aliments de Maisons-Alfort, unité des Contaminants Inorganiques et Minéraux de l’Environnement, ANSES, 23, Avenue du général de Gaulle, Maisons-Alfort, 94706, Paris, France

    Axelle Leufroy, Laurent Noël & Thierry Guérin

  2. Department of Chemistry, Queen’s University, 90 Bader Lane, Kingston, ON, K7L 3N6, Canada

    Diane Beauchemin

Authors
  1. Axelle Leufroy
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  2. Laurent Noël
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  3. Diane Beauchemin
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  4. Thierry Guérin
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Correspondence to Thierry Guérin.

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Leufroy, A., Noël, L., Beauchemin, D. et al. Bioaccessibility of total arsenic and arsenic species in seafood as determined by a continuous online leaching method. Anal Bioanal Chem 402, 2849–2859 (2012). https://doi.org/10.1007/s00216-012-5774-4

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  • DOI: https://doi.org/10.1007/s00216-012-5774-4

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