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A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology

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Abstract

One of the limits of current electrochemical biosensors is a lack of methods providing stable and highly efficient junctions between biomaterial and solid-state devices. This paper shows how laser-induced forward transfer (LIFT) can enable efficient electron transfer from photosynthetic biomaterial immobilized on screen-printed electrodes (SPE). The ideal pattern, in terms of photocurrent signal of thylakoid droplets giving a stable response signal with a current intensity of approximately 335 ± 13 nA for a thylakoid mass of 28 ± 4 ng, was selected. It is shown that the efficiency of energy production of a photosynthetic system can be strongly enhanced by the LIFT process, as demonstrated by use of the technique to construct an efficient and sensitive photosynthesis-based biosensor for detecting herbicides at nanomolar concentrations.

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Acknowledgements

The work discussed in this paper used technology developed in projects with financial support from the European Commission (e-LIFT FP7 ICT, grant agreement no. 247868; BEEP-C-EN FP 7-SME-2008-01, grant agreement no. 231082, SENSBIOSYN FP 7-SME-2008-01, grant agreement no. 232522), which is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Crystallography, National Research Council, Via Salaria Km 29.300, Monterotondo Scalo, 00015, Rome, Italy

    Eleftherios Touloupakis, Katia Buonasera & Maria Teresa Giardi

  2. Biosensor srl, Via degli Olmetti 44, Formello, 00060, Rome, Italy

    Eleftherios Touloupakis

  3. Department of Physics, National Technical University of Athens, Iroon Polytehneiou 9, Zografou, 15780, Athens, Greece

    Christos Boutopoulos & Ioanna Zergioti

Authors
  1. Eleftherios Touloupakis
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  2. Christos Boutopoulos
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  3. Katia Buonasera
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  4. Ioanna Zergioti
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  5. Maria Teresa Giardi
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Corresponding author

Correspondence to Eleftherios Touloupakis.

Additional information

Published in the topical collection Biomimetic Recognition Elements for Sensing Applications with guest editor María Cruz Moreno-Bondi.

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Touloupakis, E., Boutopoulos, C., Buonasera, K. et al. A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology. Anal Bioanal Chem 402, 3237–3244 (2012). https://doi.org/10.1007/s00216-012-5771-7

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  • DOI: https://doi.org/10.1007/s00216-012-5771-7

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