Abstract
A novel flow injection analysis (FIA) system suitable for measurement of S-nitrosothiols (RSNOs) in blood plasma is described. In the proposed (FIA) system, samples and standards containing RSNO species are injected into a buffer carrier stream that is mixed with the reagent stream containing 3,3′-dipropionicdiselenide (SeDPA) and glutathione (GSH). SeDPA has been shown previously to catalytically decompose RSNOs in the presence of a reducing agent, such as GSH, to produce nitric oxide (NO). The liberated NO is then detected downstream by an amperometric NO sensor. This sensor is prepared using an electropolymerized m-phenylenediamine (m-PD)/resorcinol and Nafion composite films at the surface of a platinum electrode. Using optimized flow rates and reagent concentrations, detection of various RSNOs at levels in the range of 0.25–20 μM is possible. For plasma samples, detection of background sensor interference levels within the samples must first be carried out using an identical FIA arrangement, but without the added SeDPA and GSH reagents. Subtraction of this background sensor current response allows good analytical recovery of RSNOs spiked into animal plasma samples, with recoveries in the range of 90.4–101.0%.
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Acknowledgments
Chuncui Huang is a visiting student from Beijing Institute of Technology and was advised by Professor Huibo Shao in China. This work was partially supported by the Science Foundation of Key Laboratory of Ministry of Education of China and the 111 Project (B07012) in China. We are indebted to the National Scholarship Fund of the China Scholarship Council for supporting visiting students. We also wish to thank the National Institutes of Health (EB-000783) for partially supporting this research.
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Huang, C., Brisbois, E. & Meyerhoff, M.E. Flow injection measurements of S-nitrosothiols species in biological samples using amperometric nitric oxide sensor and soluble organoselenium catalyst reagent. Anal Bioanal Chem 400, 1125–1135 (2011). https://doi.org/10.1007/s00216-011-4840-7
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DOI: https://doi.org/10.1007/s00216-011-4840-7