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Laser microperforated biodegradable microbial polyhydroxyalkanoate substrates for tissue repair strategies: an infrared microspectroscopy study

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Abstract

Flexible and biodegradable film substrates prepared by solvent casting from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) solutions in chloroform were microperforated by ultraviolet laser ablation and subsequently characterized using infrared (IR) microspectroscopy and imaging techniques and scanning electron microscopy (SEM). Both transmission synchrotron IR microspectroscopy and attenuated total reflectance microspectroscopy measurements demonstrate variations in the polymer at the ablated pore rims, including evidence for changes in chemical structure and crystallinity. SEM results on microperforated PHBHV substrates after cell culture demonstrated that the physical and chemical changes observed in the biomaterial did not hinder cell migration through the pores.

Composition showing visible and IR images of a microperforated PHBHV film, with IR spectra showing crystallinity differences between bulk film and pore rim, schematic of cell growth and propagation strategy and SEM image showing evidence of cell growth on the underside of the biodegradable substrate.

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Acknowledgments

Financial support was provided by the Ministerio de Ciencia e Innovación (MICINN), Instituto de Salud Carlos III, projects FIS PI05/2087 and PI08/1677. Synchrotron measurements performed at the U10B Beamline of the National Synchrotron Light Source, Brookhaven National Laboratory were supported by the US Department of Energy, Division of Materials Sciences, and Division of Chemical Sciences under Contract No. DE-AC02-98CH10886, and those performed at the SMIS Beamline of Synchrotron Soleil were supported by the European Union Trans National Access I3/IA-SFS Program (RII3-CT-2004-506008). We wish to thank the Beamline staff of both installations for their helpful support and advice.

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Authors and Affiliations

  1. Instituto de Ciencia y Tecnología de Polímeros, CSIC, Juan de la Cierva 3, 28006, Madrid, Spain

    Gary Ellis, Pilar Cano, Carlos Marco & Leoncio Garrido

  2. Instituto de Química-Física Rocasolano, CSIC, Serrano 119, 28006, Madrid, Spain

    María Jadraque & Margarita Martín

  3. Fundación Hospital de Alcorcón, Avda. Villaviciosa 2, 28922, Alcorcón, Spain

    Laura López, Teresa Núñez & Enrique de la Peña

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  1. Gary Ellis
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  2. Pilar Cano
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  3. María Jadraque
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Correspondence to Gary Ellis.

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Ellis, G., Cano, P., Jadraque, M. et al. Laser microperforated biodegradable microbial polyhydroxyalkanoate substrates for tissue repair strategies: an infrared microspectroscopy study. Anal Bioanal Chem 399, 2379–2388 (2011). https://doi.org/10.1007/s00216-011-4653-8

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