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Discriminating olive and non-olive oils using HPLC-CAD and chemometrics

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Abstract

This work presents a method for an efficient differentiation of olive oil and several types of vegetable oils using chemometric tools. Triacylglycerides (TAGs) profiles of 126 samples of different categories and varieties of olive oils, and types of edible oils, including corn, sunflower, peanut, soybean, rapeseed, canola, seed, sesame, grape seed, and some mixed oils, have been analyzed. High-performance liquid chromatography coupled to a charged aerosol detector was used to characterize TAGs. The complete chromatograms were evaluated by PCA, PLS-DA, and MCR in combination with suitable preprocessing. The chromatographic data show two clusters; one for olive oil samples and another for the non-olive oils. Commercial oil blends are located between the groups, depending on the concentration of olive oil in the sample. As a result, a good classification among olive oils and non-olive oils and a chemical justification of such classification was achieved.

PCA scores plot of oil samples. Olive oils (asterisk), non-olive oils (filled square) and oil blends (filled inverse triangle)

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Acknowledgments

The authors acknowledge the Andalusia Regional Government (Consejería de Innovación, Ciencia y Empresa, project P07-FQM-02667) for financial assistance. This work has also been partially supported by European Regional Development Funds. In addition, one of the authors, PME, acknowledges the postgraduate grant to the Consejo Nacional de Ciencia y Tecnología, México (CONACYT).

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Authors and Affiliations

  1. Department of Analytical Chemistry, Unit of Chemical Metrology and Qualimetrics (CMQ), University of Granada, 18071, Granada, Spain

    P. de la Mata-Espinosa, J. M. Bosque-Sendra & L. Cuadros-Rodríguez

  2. Quality & Technology, Department of Food Science, Faculty of Life Sciences, University of Copenhagen, 1165, Copenhagen, Denmark

    R. Bro

Authors
  1. P. de la Mata-Espinosa
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  2. J. M. Bosque-Sendra
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  3. R. Bro
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  4. L. Cuadros-Rodríguez
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Correspondence to J. M. Bosque-Sendra.

Additional information

Published in the special issue Chemometrics (VII Colloquium Chemiometricum Mediterraneum) with Guest Editors Marcelo Blanco, Juan M. Bosque-Sendra, and Luis Cuadros-Rodríguez.

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de la Mata-Espinosa, P., Bosque-Sendra, J.M., Bro, R. et al. Discriminating olive and non-olive oils using HPLC-CAD and chemometrics. Anal Bioanal Chem 399, 2083–2092 (2011). https://doi.org/10.1007/s00216-010-4366-4

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  • DOI: https://doi.org/10.1007/s00216-010-4366-4

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