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Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120

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Abstract

We evaluated the potential of a quartz crystal microbalance with dissipation monitoring (QCM-D) to provide a sensitive, label-free method for detecting the conformational rearrangement of glycoprotein gp120 upon binding to different ligands. This glycoprotein is normally found on the envelope of the HIV-1 virus and is involved in viral entry into host cells. It was immobilized on the surface of the sensing element of the QCM-D and was exposed to individual solutions of several different small-molecule inhibitors as well as to a solution of a soluble form of the host cell receptor to which gp120 binds. Instrument responses to ligand-triggered changes were in qualitative agreement with conformational changes as suggested by other biophysical methods.

Graphic to accompany the on-line abstract for "Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120," by Hyun-Su Lee, Mark Contarino, M. Umashankara, Arne Schön, Ernesto Freire, Amos B. Smith, III, Irwin M. Chaiken, and Lynn S. Penn

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Acknowledgments

The authors thank Dr. Jason Cox, Department of Chemistry, University of Pennsylvania, for supplying BMS-806. They also thank the National Institutes of Health 5P01GM56550-12, National Institutes of Health R21AI071965-01, and International Partnership for Microbicides/USAID for funding.

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Authors and Affiliations

  1. Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA, 19104, USA

    Hyun-Su Lee & Lynn S. Penn

  2. Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA, 19102, USA

    Mark Contarino, M. Umashankara & Irwin M. Chaiken

  3. Department of Biology, Johns Hopkins University, 3400 N. Charles Street, Baltimore, MD, 21218, USA

    Arne Schön & Ernesto Freire

  4. Department of Chemistry, University of Pennsylvania, 231 S. 34th Street, Philadelphia, PA, 19104, USA

    Amos B. Smith III

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  1. Hyun-Su Lee
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Correspondence to Lynn S. Penn.

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Lee, HS., Contarino, M., Umashankara, M. et al. Use of the quartz crystal microbalance to monitor ligand-induced conformational rearrangements in HIV-1 envelope protein gp120. Anal Bioanal Chem 396, 1143–1152 (2010). https://doi.org/10.1007/s00216-009-3313-8

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  • DOI: https://doi.org/10.1007/s00216-009-3313-8

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