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Fiber-packed SPE tips based on electrospun fibers

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Abstract

A novel fiber-packed solid-phase extraction (SPE) tip was designed based on electrospun nanofibers. The tip was used to investigate the extraction of hydrocortisone (HC), cortisone acetate (CA), ethinylestradiol (EE), and estradiol (E2). The effects of diameters, porous figurations, and functional groups of the electrospun fibers on the selectivity and efficiency were studied. The experimental results indicated that the detection limit of cortisol in water sample could be as low as 0.75 ng/mL. When the tip is used for detection of cortisol in human hair the efficiency of biological sample pretreatment is better than the traditional SPE method. Our method could significantly simplify the traditional SPE process and lower the cost. Industrial application of the tip is anticipated.

Analyte molecules (e.g., cortisol) are attracted onto the electrospun nanofibers of the packed SPE tip. Careful selection of the fiber diameter, morphology, and functional groups affords high selectivity, sensitivity, and extraction recoveries

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References

  1. McDowall RD (1989) J Chromatogr B 492:3–58

    Article  CAS  Google Scholar 

  2. Kang KJ, Pan C, Xu Q, Yao YF, Gu ZZ (2007) Aanl Chim Acta 587:75–81

    Article  CAS  Google Scholar 

  3. Fridrikh SV, Yu JH, Brenner MP, Rutledge GC (2003) Phys Rev Lett 90:144502 /144501–4

    Article  Google Scholar 

  4. Stankusa JJ, Guan J, Fujimotoc K, Wagner WR (2006) Biomaterials 27:735–744

    Article  Google Scholar 

  5. Casper CL, Stephens JS, Tassi NG (2004) Macromolecules 37:573–578

    Article  CAS  Google Scholar 

  6. Megelski S, Stephens JS, Chase DB, Rabolt JF (2002) Macromolecules 35:8456–8466

    Article  CAS  Google Scholar 

  7. Casper CL, Stephens JS, Tassi NG, Chase DB, Rabolt JF (2004) Macromolecules 37:573–578

    Article  CAS  Google Scholar 

  8. Tiosano D, Eisentein I, Militianu D, Chrousos GP, Hochberg Z (2003) J Clin Endocr Metab 88:379–384

    Article  CAS  Google Scholar 

  9. Reincke M (2000) Endocrinol Metab Clin North Am 29:43–56

    Article  CAS  Google Scholar 

  10. Sandeep TC, Walker BR (2001) Trends Endocrinol Metab 12:446–453

    Article  CAS  Google Scholar 

  11. Edwards CR, Benediktsson R, Lindsay RS, Seckl JR (1996) Steroids 61:263–269

    Article  CAS  Google Scholar 

  12. Kushnir MM, Neilson R, Roberts WL, Rockwood AL (2004) Clin Biochem 37:357–362

    Article  CAS  Google Scholar 

  13. Manami N, Mikio Y, Akira N, Kojiro K (2006) J Chromatogr B 842:106–110

    Article  Google Scholar 

  14. Baumgartner W, Hill V (1996) In: Kintz P (ed) Drug testing in hair. CRC, Boca Raton, pp 223–266

  15. Bevalot F, Gaillard Y, Lhermitte MA, Pep’in G (2000) J Chromatogr B 740:227–236

    Article  CAS  Google Scholar 

  16. De Boer HS, Lansaat PH, Kooistra KR, Van Oort WJ (1979) Anal Chim Acta 111:275–279

    Article  Google Scholar 

  17. Santos MA, Gonzalo LR, Gasco LA, Izquierdo HR (1994) J Chromatogr B 652:83–89

    Article  Google Scholar 

  18. Gu ZZ, Chen HH, Zhang S, Sun LG, Xie ZY (2007) Colloid Surf A 302:312–319

    Article  CAS  Google Scholar 

  19. Gu ZZ, Wei HM, Zhang RQ (2005) Appl Phys Lett 86:201915 /1–3

    Article  Google Scholar 

  20. Qi DJ, Kang XJ, Chen LQ, Zhang YY, Wei HM, Gu ZZ (2008) Anal Bioanal Chem (in press)

  21. Be’valot F, Gaillard Y, Lhermitte MA, Pe’pin G (2000) J Chromatogr B 740:227–236

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was supported by Jiangsu Science and Technology Department (Grant No. BK2006509), National Natural Science Foundation of China (Grant No. 60121101).

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, Southeast University, Si Pai Lou 2, Nanjing, 210096, China

    Yiyun Zhang, Chao Pan, Yingfang Yao & Zhong-Ze Gu

  2. Lab of Environment and Biosafety, Research Institute of Southeast University in Suzhou, Dushu Lake Higher Education Town, Suzhou, 215123, China

    Xuejun Kang & Zhong-Ze Gu

  3. Key Laboratory of Child Development and Learning Science (Ministry of Education), Research Center for Learning Science, Southeast University, Si Pai Lou 2, Nanjing, 210096, China

    Xuejun Kang & Liqin Chen

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  1. Yiyun Zhang
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  2. Xuejun Kang
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  3. Liqin Chen
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  4. Chao Pan
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  5. Yingfang Yao
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Correspondence to Zhong-Ze Gu.

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Zhang, Y., Kang, X., Chen, L. et al. Fiber-packed SPE tips based on electrospun fibers. Anal Bioanal Chem 391, 2189–2197 (2008). https://doi.org/10.1007/s00216-008-2145-2

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  • DOI: https://doi.org/10.1007/s00216-008-2145-2

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