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Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis

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Abstract

Total selenium content and its distribution in the soluble and insoluble protein-bound fractions obtained after aqueous extraction of antarctic krill samples were determined. About 26% of the total selenium (2.4 μg g−1 dry weight) was found in the supernatant; the rest was in the pellet. Isolation of low molecular selenium-containing fractions was also performed by enzymatic digestion of the protein, followed by size-exclusion chromatography in conjunction with atomic absorption spectrometry. From the applied various proteinases (pronase E, subtilisin Carlsberg, trypsin, chymotrypsin, proteinase and proteinase N from Bacillus subtilis and Novo 0.6 MPX enzyme), the treatment with pronase E led to best recovery of selenium. About 96% of the total Se was found in the hydrolysate, mainly in low molecular weight fractions. Eighty percent of the Se species were in fractions with molecular weights in the range of amino acids and short peptides. High-performance liquid chromatography/inductively coupled plasma mass spectrometry (HPLC-ICP-MS) allowed the identification of selenomethionine and the assumption that selenocystine or its derivatives were the main species in these fractions.

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Acknowledgements

The authors would like to thank Drs. H. Helmholz and J. Bergmann (Institute for Coastal Research, GKSS Geesthacht) for the discussions and the support in HPLC-ICP-MS analysis, Mr. V. Klatt and Mr. V. Block (Central Analytical Laboratory of the Technical University Hamburg-Harburg) for their assistance in GF-AAS analysis. This work has been financially supported by GKSS Research Centre, Geestacht, fund for Cooperation with Universities (Hochschulzusammenarbeit).

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Authors and Affiliations

  1. Institute of Biotechnology II, Technical University Hamburg-Harburg, Denickestrasse 15, 21071, Hamburg, Germany

    Mariana Siwek & Boris Galunsky

  2. Institute for Coastal Research, GKSS Research Centre, Max-Planck-Strasse 1, 21502, Geesthacht, Germany

    Bernd Niemeyer

  3. Institute of Thermodynamics, Helmut-Schmidt-University/University of the Federal Armed Forces Hamburg, Hostenhofweg 85, 22043, Hamburg, Germany

    Bernd Niemeyer

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  1. Mariana Siwek
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  2. Boris Galunsky
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  3. Bernd Niemeyer
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Correspondence to Mariana Siwek.

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Siwek, M., Galunsky, B. & Niemeyer, B. Isolation of selenium organic species from antarctic krill after enzymatic hydrolysis. Anal Bioanal Chem 381, 737–741 (2005). https://doi.org/10.1007/s00216-004-2936-z

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  • DOI: https://doi.org/10.1007/s00216-004-2936-z

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