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Fast LC/MS in the analysis of small molecules

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Abstract

Liquid chromatography-mass spectrometry (LC/MS) has become one of the most widely used analytical techniques in both qualitative and quantitative analysis of small molecules. Recently, with the increasing demand for ever-higher sample throughput, the use of faster chromatographic separations has become popular, along with other LC/MS methods that decrease analytical cycle-time. The burgeoning use of LC/MS has meant that the primary expertise of many practitioners today is not in the field of LC/MS, which has been facilitated by the ease-of-use of modern LC/MS systems. An examination of the current state of the literature, relating to "fast LC/MS", should serve well to those new to LC/MS, and should help them in the development of fast LC/MS methods that are effective in terms of both the chromatography and the utilization of the mass spectrometer. This review paper focuses on fast LC/MS analyses of small molecules that have been reported in peer-reviewed publications.

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Authors and Affiliations

  1. Drug Metabolism, Merck Research Laboratories, West Point, PA 19486, USA

    Philip R. Tiller

  2. Drug Metabolism, Merck Research Laboratories, Rahway, NJ 07065, USA

    Leslie A. Romanyshyn

  3. Waters Corporation, Milford, MA 01757, USA

    Uwe D. Neue

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  1. Philip R. Tiller
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  2. Leslie A. Romanyshyn
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  3. Uwe D. Neue
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Correspondence to Philip R. Tiller.

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Tiller, P.R., Romanyshyn, L.A. & Neue, U.D. Fast LC/MS in the analysis of small molecules. Anal Bioanal Chem 377, 788–802 (2003). https://doi.org/10.1007/s00216-003-2146-0

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  • DOI: https://doi.org/10.1007/s00216-003-2146-0

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