Abstract
The aim of this study is to investigate the therapeutic effects of vitamin U (Vit U) on lung tissue of pentyleneterazole (PTZ)-induced seizures in rats. Sprague Dawley male rats were randomly divided into four groups as follows: control (0.9% NaCl given, intraperitoneally); Vit U (50 mg/kg/day, for 7 days by gavage); PTZ; (60 mg/kg one dose, intraperitoneally); and PTZ + Vit U (in same dose and time). At the end of the experiment, lung tissues were taken and examined biochemically and cytologically. Lipid peroxidation (LPO), glutathione (GSH), sialic acid (SA), and nitric oxide (NO) levels, and superoxide dismutase (SOD) and catalase (CAT) activities were determined in lung homogenates. Imprinted lung samples were stained with May Grunwald-Giemsa stain and microscopically examined for the presence of collagen fibers, macrophage, leucocyte, and epithelial cells. PTZ administration significantly increased GSH level and CAT activity and significantly decreased SOD activity compared to the control group. Vit U administration significantly increased GSH level and CAT activity compared to the control group. GSH and NO levels significantly decreased in PTZ + Vit U group compared to the PTZ group. In cytologic analysis, increased collagen fibers, macrophages, leucocytes, and epithelial cells were observed in PTZ group compared to the control group, and Vit U administration decreased these cytological parameters compared to the PTZ group. The findings of this study support the possible protective role of using Vit U as an add-on therapy in order to prevent lung tissue injury which may occur during seizures in epilepsy.
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References
Acharya MM, Hattiangady B, Shetty AK (2008) Progress in neuroprotection strategies for preventing epilepsy. Prog Neurobiol 84(4):363–404. https://doi.org/10.1016/j.pneurobio.2007.10.010
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Aguiar CCT, Almeida AB, Araújo PVP, Abreu RNDCD, Chaves EMC, Vale OCD, Macedo DS, Woods DJ, Fonteles MMDF, Vasconcelos SMM (2012) Oxidative stress and epilepsy: literature review. Oxidative Med Cell Longev 2012:1–12. https://doi.org/10.1155/2012/795259
Atay Z, Topalidis T (1992) Cytodiagnostic der serosen hohlen. Atlas und Lehrbuch. Herausgeber; A&T Hannover: Wolfgang Pabst Verlag, pp: 18–19
Barnham KJ, Masters CL, Bush AI (2004) Neurodegenerative diseases and oxidative stress. Nat Rev Drug Discov 3(3):205–214. https://doi.org/10.1038/nrd1330
Belcastro V, Striano P (2012) Antiepileptic drugs, hyperhomocysteinemia and B-vitamins supplementation in patients with epilepsy. Epilepsy Res 102(1-2):1–7. https://doi.org/10.1016/j.eplepsyres.2012.07.003
Bellissimo MI, Amado D, Abdalla DS, Ferreira EC, Cavalheiro EA, Naffah-Mazzacoratti MG (2001) Superoxide dismutase, glutathione peroxidase activities and the hydroperoxide concentration are modified in the hippocampus of epileptic rats. Epilepsy Res 46(2):121–128. https://doi.org/10.1016/S0920-1211(01)00269-8
Beutler E (1975) Glutathione: red cell metabolism. A manual biochemical methods. Grune and Stratton, New York, pp 112–114
Cardenas-Rodriguez N, Huerta-Gertrudis B, Rivera-Espinosa L, Montesinos-Correa H, Bandala C, Carmona-Aparicio L, Coballase-Urrutia E (2013) Role of oxidative stress in refractory epilepsy: evidence in patients and experimental models. Int J Mol Sci 14(1):1455–1476. https://doi.org/10.3390/ijms14011455
Dey A, Kang X, Qui J, Du Y, Jiang J (2016) Anti-inflammatory small molecules to treat seizures and epilepsy: from bench to bedside. Trends Pharmacol Sci 37(6):463–484. https://doi.org/10.1016/j.tips.2016.03.001
Dillioglugil MO, Kir HM, Demir C, Ilbay G, Sahin D, Dillioglugil O, Bambal G, Mekik H, Ates N (2010) Effect of pentylenetetrazole and sound stimulation induced single and repeated convulsive seizures on the MDA, GSH and NO levels, and SOD activities in rat liver and kidney tissues. Brain Res Bull 83(6):356–359. https://doi.org/10.1016/j.brainresbull.2010.09.007
Diniz TC, Silva JC, de Lima-Saraiva SR, Ribeiro FP, Pacheco AG, de Freitas RM, Quintans-Júnior LJ, Quintas Jde S, Mendes RL, Almeida JR (2015) The role of flavonoids on oxidative stress in epilepsy. Oxidative Med Cell Longev 2015:1–9. https://doi.org/10.1155/2015/171756
Esmekaya MA, Tuysuz MZ, Tomruk A, Canseven AG, Yücel E, Aktuna Z, Keskil S, Seyhan N (2016) Effects of cell phone radiation on lipid peroxidation, glutathione and nitric oxide levels in mouse brain during epileptic seizure. J Chem Neuroanat 75(Pt B):111–115. https://doi.org/10.1016/j.jchemneu.2016.01.011
Gezginci-Oktayoglu S, Turkyilmaz IB, Ercin M, Yanardag R, Bolkent S (2016) Vitamin U has a protective effect on valproic acid-induced renal damage due to its anti-oxidant, anti-inflammatory, and anti-fibrotic properties. Protoplasma 253(1):127–135. https://doi.org/10.1007/s00709-015-0796-3
Goldman AM (2015) Mechanisms of sudden unexplained death in epilepsy. Curr Opin Neurol 28(2):166–174. https://doi.org/10.1097/WCO.0000000000000184
Greene BR, De Chazal P, Boylan G, ReilLowly RB, O’Brien C, Connolly S (2006) Heart and respiration rate changes in the neonate during electroencephalographic seizure. MBEC 44(1-2):27–34. https://doi.org/10.1007/s11517-005-0001-5
Hamed SA, Abdellah MM, El-Melegy N (2004) Blood levels of trace elements, electrolytes, and oxidative stress/antioxidant systems in epileptic patients. JPS 96:465–473
Harden C, Tomson T, Gloss D, Buchhalter J, Cross JH, Donner E, French JA, Gil-Nagel A, Hesdorffer DC, Smithson WH, Spitz MC, Walczak TS, Sander JW, Ryvlin P (2017) Practice guideline summary: sudden unexpected death in epilepsy incidence rates and risk factors Report of the Guideline Development, Dissemination, and Implementation Subcommittee of the American Academy of Neurology and the American Epilepsy Society. Neurology 88(17):1674–1680. https://doi.org/10.1212/WNL.0000000000003685
Ho YH, Lin YT, Wu CWJ, Chao YM, Chang AY, Chan JY (2015) Peripheral inflammation increases seizure susceptibility via the induction of neuroinflammation and oxidative stress in the hippocampus. J Biomed Sci 22:46. https://doi.org/10.1186/s12929-015-0157-8
Holtman L, van Vliet EA, Aronica E, Wouters D, Wadman WJ, Gorter JA (2013) Blood plasma inflammation markers during epileptogenesis in post-status epilepticus rat model for temporal lobe epilepsy. Epilepsia 54(4):589–595. https://doi.org/10.1111/epi.12112
Hu BL, Hou WC, Li XP, Ma LJ, Bai XX, Liu Q, Liu BH (2017) Aster tataricus attenuates the neuronal cell damage and restores the cognitive functions in epileptic rat. Biomed Res 28:1189–1194
Ilhan A, Ahmet G, Ferah A, Suat K, Mustafa I (2005a) Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazole induced kindling in mice. Neuropharmacology 49(4):456–464. https://doi.org/10.1016/j.neuropharm.2005.04.004
Ilhan A, Aladag MA, Kocer A, Boluk A, Gurel A, Armutcu F (2005b) Erdosteine ameliorates PTZ-induced oxidative stress in mice seizure model. Brain Res Bull 65(6):495–499. https://doi.org/10.1016/j.brainresbull.2005.02.027
Kang HC, Lee YM, Kim HD (2013) Mitochondrial disease and epilepsy. Brain and Development 35(8):757–761. https://doi.org/10.1016/j.braindev.2013.01.006
Kong Q, Lin CLG (2010) Oxidative damage to RNA: mechanisms, consequences, and diseases. Cell Mol Life Sci 67(11):1817–1829. https://doi.org/10.1007/s00018-010-0277-y
Kovac S, Dinkova-Kostova AT, Abramov AY (2016) The role of reactive oxygen species in epilepsy. ROS 1:38–52
Kroner BL, Fahimi M, Gaillard WD, Kenyon A, Thurman DJ (2016) Epilepsy or seizure disorder? The effect of cultural and socioeconomic factors on self-reported prevalence. Epilepsy Behav 62:214–217. https://doi.org/10.1016/j.yebeh.2016.07.013
Kubova H (2016) Side effects of antiepileptic drugs. Antiepileptic Drug Discovery: Novel Approaches. Humana Press, New York, pp 329–350
Lee SW, Chung SS (2010) A review of the effects of vitamins and other dietary supplements on seizure activity. Epilepsy Behav 18(3):139–150. https://doi.org/10.1016/j.yebeh.2010.04.013
Lee NY, Park KY, Min HJ, Song KY, Lim YY, Park J, Kim BJ, Kim MN (2012) Inhibitory effect of vitamin U (S-methylmethionine sulfonium chloride) on differentiation in 3T3-L1 pre-adipocyte cell lines. Ann Dermatol 24(1):39–44. https://doi.org/10.5021/ad.2012.24.1.39
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265–275
Martinc B, Grabnar I, Vovk T (2014) Antioxidants as a preventive treatment for epileptic process: a review of the current status. Curr Neuropharmacol 12(6):527–550. https://doi.org/10.2174/1570159X12666140923205715
Mehvari J, Motlagh FG, Najafi M, Ghazvini MRA, Naeini AA, Zare M (2016) Effects of Vitamin E on seizure frequency, electroencephalogram findings, and oxidative stress status of refractory epileptic patients. Adv Biomed Res 5(1):36. https://doi.org/10.4103/2277-9175.178780
Méndez-Armenta M, Nava-Ruíz C, Juárez-Rebollar D, Rodríguez-Martínez E, Gómez PY (2014) Oxidative stress associated with neuronal apoptosis in experimental models of epilepsy. Oxidative Med Cell Longev 2014:1–12. https://doi.org/10.1155/2014/293689
Miranda KM, Espey MG, Wink DA (2001) A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 5(1):62–71. https://doi.org/10.1006/niox.2000.0319
Morimoto M, Satomura S, Hashimoto T, Ito E, Kyotani S (2016) Oxidative stress measurement and prediction of epileptic seizure in children and adults with severe motor and intellectual disabilities. J Clin Med Res 8(6):437–444. 10.14740/jocmr2534w
Mylorie AA, Collins H, Umbles C, Kyle J (1986) Erythrocyte SOD activity and other parameters of copper status in rats ingesting lead acetate. Toxicol Appl Pharmacol 82(3):512–520. https://doi.org/10.1016/0041-008X(86)90286-3
O’regan ME, Brown JK (2005) Abnormalities in cardiac and respiratory function observed during seizures in childhood. Dev Med Child Neurol 47(1):4–9
Patel AD, Prajapati NK (2012) Review on biochemical importance of Vitamin U. J Chem Pharm Res 4:209–215
Patil S, Katolkar U, Salve A, Mundhada D (2017) The neuroprotective effect of α-tocopherol on ptz-induced epilepsy causes oxidative stress and memory impairment in rats. WJPPS 6:1124–1140
Pearson-Smith JN, Liang LP, Rowley SD, Day BJ, Patel M (2017) Oxidative stress contributes to status epilepticus associated mortality. Neurochem Res 42(7):2024–2032. https://doi.org/10.1007/s11064-017-2273-1
Racine RJ (1972) Modification of seizure activity by electrical stimulation. II Motor seizure EEG 32:281–294
Rasic-Markovic A, Hrncic D, Krstić D, Colovic M, Djuric E, Rankov-Petrović B, Susic V, Stanojlovic O, Djuric D (2016) The effect of subchronic supplementation with folic acid and l-arginine on homocysteine induced seizures. Can J Physiol Pharmcol 94(10):1083–1089. https://doi.org/10.1139/cjpp-2016-0076
Riazi K, Galic MA, Pittman QJ (2010) Contributions of peripheral inflammation to seizure susceptibility: cytokines and brain excitability. Epilepsy Res 89(1):34–42. https://doi.org/10.1016/j.eplepsyres.2009.09.004
Rowley S, Patel M (2013) Mitochondrial involvement and oxidative stress in temporal lobe epilepsy. Free Radic Biol Med 62:121–131. https://doi.org/10.1016/j.freeradbiomed.2013.02.002
Selassie AW, Wilson DA, Martz GU, Smith GG, Wagner JL, Wannamaker BB (2014) Epilepsy beyond seizure: a population-based study of comorbidities. Epilepsy Res 108(2):305–315. https://doi.org/10.1016/j.eplepsyres.2013.12.002
Sokmen BB, Tunali S, Yanardag R (2012) Effects of vitamin U (S-methyl methionine sulphonium chloride) on valproic acid induced liver injury in rats. Food Chem Toxicol 50(10):3562–3566. https://doi.org/10.1016/j.fct.2012.07.056
Speakman RJ (2003) Oxidative phosphorylation, mitochondrial proton cycling, free-radical production and aging. Adv Cell Aging Gerontol 14:35–68. https://doi.org/10.1016/S1566-3124(03)14003-5
Tambe R, Jain P, Patil S, Ghumatkar P, Sathaye S (2016) Antiepileptogenic effects of borneol in pentylenetetrazole-induced kindling in mice. N-S. Arch Pharmacol 389(5):467–475. https://doi.org/10.1007/s00210-016-1220-z
Tunali S, Kahraman S, Yanardag R (2015) Vitamin U, a novel free radical scavenger, prevents lens injury in rats administered with valproic acid. Hum Exp Toxicol 34(9):904–910. https://doi.org/10.1177/0960327114561665
Vercueil L (2006) Epilepsy and neurodegenerative diseases in adults: a clinical review. Epileptic Disord 8:44–54
Vezzani A (2014) Epilepsy and inflammation in the brain: overview and pathophysiology. Epilepsy Curr 14(s2):3–7. https://doi.org/10.5698/1535-7511-14.s2.3
Vezzani A, Balosso S, Ravizza T (2008) The role of cytokines in the pathophysiology of epilepsy. Brain Behav Immun 22(6):797–803. https://doi.org/10.1016/j.bbi.2008.03.009
Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chem 234(8):1971–1975
Yagi K (1981) Assay for blood plasma or serum. Methods Enzymol 105:328–337
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This work was supported by the Scientific Research Project Department of Marmara University (Project No. SAG-D-071015-0475).
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Oktay, S., Bayrak, G., Alev, B. et al. The effect of vitamin U on the lung tissue of pentyleneterazole-induced seizures in rats. Naunyn-Schmiedeberg's Arch Pharmacol 391, 177–184 (2018). https://doi.org/10.1007/s00210-017-1447-3
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DOI: https://doi.org/10.1007/s00210-017-1447-3