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β-Glucan extracted from the medicinal mushroom Agaricus blazei prevents the genotoxic effects of benzo[a]pyrene in the human hepatoma cell line HepG2

  • Genotoxicity and Carcinogenicity
  • Published:
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Abstract

The mushroom Agaricus blazei is studied for its nutraceutical potential and as a medicinal supplement. The aim of the present study was to investigate the chemoprotective effect of β-glucan extracted from the mushroom A. blazei against DNA damage induced by benzo[a]pyrene (B[a]P), using the comet assay (genotoxicity) and micronucleus assay with cytokinesis block (mutagenicity) in a human hepatoma cell line (HepG2). To elucidate the possible β-glucan mechanism of action, desmutagenesis or bioantimutagenesis types, three treatment protocols were tested: simultaneous, pre-treatment, and presimultaneous. The results showed that β-glucan does not exert genotoxic or mutagenic effect, but that it does protect against DNA damage caused by B[a]P in every protocol tested. The data suggest that β-glucan acts through binding to B[a]P or the capture of free radicals produced during its activation. On the other hand, the pre-treatment results also suggest the possibility that β-glucan modulates cell metabolism.

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Acknowledgments

We thank FAEPE/UEL, CNPq, and CAPES for financial support and grants. We also thank Prof. Sandra S. Soares for donating the samples of β-glucan. We are grateful to Dr A. Leyva for English editing of the manuscript.

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Authors and Affiliations

  1. Laboratório de Genética Toxicológica, Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, Paraná, 86951-990, Brazil

    José Pedro Friedmann Angeli, Marilanda Ferreira Bellini & Mário Sérgio Mantovani

  2. Programa de Pós-Graduação em Patologia, Faculdade de Medicina, UNESP, Botucatu, Sao Paulo, Brazil

    Lúcia Regina Ribeiro

  3. Programa de Pós-Graduação em Biologia Celular e Molecular, Depto. de Biologia, UNESP, Rio Claro, Sao Paulo, Brazil

    Lúcia Regina Ribeiro

Authors
  1. José Pedro Friedmann Angeli
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  2. Lúcia Regina Ribeiro
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  3. Marilanda Ferreira Bellini
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  4. Mário Sérgio Mantovani
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Correspondence to Mário Sérgio Mantovani.

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Angeli, J.P.F., Ribeiro, L.R., Bellini, M.F. et al. β-Glucan extracted from the medicinal mushroom Agaricus blazei prevents the genotoxic effects of benzo[a]pyrene in the human hepatoma cell line HepG2. Arch Toxicol 83, 81–86 (2009). https://doi.org/10.1007/s00204-008-0319-5

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  • DOI: https://doi.org/10.1007/s00204-008-0319-5

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