Abstract
It is known that co-cultivation of green algae with heterotrophic microorganisms, such as yeast, improves green algae's growth potential and carbon dioxide fixation, even under low CO2 concentration conditions such as the atmosphere. Introducing mutations into green algae is also expected to enhance their growth potential. In this study, we sought to improve the growth potential of a co-culture system of the green algae Chlamydomonas reinhardtii and the yeast Saccharomyces cerevisiae by introducing mutations into the green algae. Additionally, we performed a transcriptome analysis of the co-culture of the green algae mutant strain with yeast, discussing the interaction between the green algae mutant strain and the yeast. When the green algae mutant strain was co-cultured with yeast, the number of green algae cells reached 152 × 105 cells/mL after 7 days of culture. This count was 2.6 times higher than when the wild-type green algae strain was cultured alone and 1.6 times higher than when the wild-type green algae strain and yeast were co-cultured. The transcriptome analysis also indicated that the primary reason for the increased growth potential of the green algae mutant strain was its enhanced photosynthetic activity and nitrogen utilization efficiency.
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The data supporting the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
The green alga, C. reinhardtii NIES-2238, was provided by NIES through the NBRP of MEXT (Japan). We would like to thank Editage for the English language editing.
Funding
This study was partially supported by the Japan Society for the Promotion of Science (Grant Number: JP22H03803) and the Kurata Grants by The Hitachi Global Foundation.
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YK: investigation, writing–original draft; RY: conceptualization, writing–original draft, writing–review and editing, supervision, funding acquisition; TM and HO: supervision.
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Communicated by Nischitha R.
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Karitani, Y., Yamada, R., Matsumoto, T. et al. UV mutagenesis improves growth potential of green algae in a green algae–yeast co-culture system. Arch Microbiol 206, 61 (2024). https://doi.org/10.1007/s00203-023-03796-2
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DOI: https://doi.org/10.1007/s00203-023-03796-2