Abstract
Using the culturomics approach, the previously unknown strain Marseille-P8953T, was isolated and classified within the Weizmannia genus. Strain Marseille-P8953T was isolated from the faeces of a healthy subject and consisted of Gram-stain positive, spore-forming, motile rod-shaped cells. A 99.2% similarity was observed between the 16S rRNA gene of strain Marseille-P8953T (accession number LR735539) and that of Weizmannia coagulans strain NBRC 12583T (accession number KX261624), its closest phylogenetic relative, while the genome of strain Marseille-P8953T (3.5 Mpb long, 46.5% GC content) shared the average nucleotide identity by Orthology and digital DNA-DNA Hybridisation values of 95 and 60.4%, respectively. Given the phylogenetic classification and phenotypic characteristics of strain Marseille-P8953T, we propose the creation of a new species within the Weizmannia genus named Weizmannia faecalis (= CSUR P8953T = CECT 9904 T).
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Abbreviations
- ANI:
-
Nucleotide identity
- COGs:
-
Cluster orthologous groups
- dDDH:
-
Digital DNA-DNA hybrydation
- FAME:
-
Fatty acid methyl ester
- GBDP:
-
Genome BLAST distance phylogeny approach
- GC/MS:
-
Gaz chromatography/mass spectrometry
- gDNA:
-
Genomic DNA
- MALDI–TOF MS:
-
Matrix assisted laser desorption ionization–Time of flight mass stpectrometry
- MIC:
-
Minimum inhibitory concentration
- OAT:
-
Orthologous ANI tool
- ORFs:
-
Open reading frames
- OrthoANI:
-
Nucleotide identity by orthology
- PHAST:
-
PHAge search tool
- RAST:
-
Rapid annotation using subsystem technology
- TMHMM:
-
Transmembrane helices hidden markov models
- TYGS:
-
Type (strain) genome server
- UPGMA:
-
Unweighted pair group method with arithmetics
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Funding
This study was funded by the Institut Hospitalo-Universitaire (IHU) Méditerranée Infection Foundation, under the program “Investissement d’avenir” (reference: ANR-10-IAHU-03) of the Région Provence Alpes Côte d’Azur and European funding FEDER PRIMI.
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Conceptualisation: DR, MTA. Formal analysis: HTK, LB, NA, PD. Funding acquisition: DR. Investigation: HTK, TPTP. Resources: SA, AD, CS. Supervision: JCL, DR, CIL, MTA. Validation: JCL, DR, MTA. Writing-original draft: HTK, MTA. Writing-review and editing: MTA, DR.
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The studies were approved by the national ethics committee of Senegal under protocol number SEN16/45 and the ethics committee of the Institut Hospitalo-Universitaire (IHU) Méditerranée Infection under agreement number 2017–009.
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Communicated by Erko Stackebrandt.
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Kieu, H.T., Pham, T.P.T., Lo, C.I. et al. Weizmannia faecalis sp. nov., isolated from a human stool sample. Arch Microbiol 204, 612 (2022). https://doi.org/10.1007/s00203-022-03229-6
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DOI: https://doi.org/10.1007/s00203-022-03229-6