Abstract
A Gram-stain-negative, motile by gliding, catalase positive, facultative anaerobic strain, designated strain XSD401T, was isolated from an unidentified Gelidium species of Xiaoshi Island, Shandong Province, China. The 16S rRNA gene sequence comparisons indicated strain XSD401T had a sequence similarity of 96.9% with Psychroserpens damuponensis KCTC 23539T and 96.3% with Psychroserpens burtonensis DSM 12212T. The G + C content of the genomic DNA was 33.9%. The ANI values between strain XSD401T and P. damuponensis KCTC 23539T, P. burtonensis DSM 12212T, were 76.9% and 76.9%, respectively. The dDDH values between strain XSD401T and P. damuponensis KCTC 23539T, P. burtonensis DSM 12212T, were 20.4% and 20.3%, respectively. The AAI values and POCP values of these 8 species were all over 72% and 50%. Combined with the results of comparative genomic analysis, Ichthyenterobacterium magnum, Flavihalobacter algicola and Arcticiflavibacter luteus were reclassified into Psychroserpens. Furthermore, the differences in morphology, physiology and genotype from the previously described taxa support the classification of strain XSD401T as a representative of the genus Psychroserpens, for which the name Psychroserpens luteolus sp. nov. is proposed. The type strain is XSD401T (= MCCC 1H00396T = KCTC 72684T = JCM 33931T).
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Abbreviations
- KCTC:
-
Korean Collection for Type Cultures
- JCM:
-
Japan Collection of Microorganisms
- MCCC:
-
Marine culture collection of China
- MEGA:
-
Molecular evolutionary genetics analysis
- dDDH:
-
The digital DNA–DNA hybridisation
- ANI:
-
Average nucleotide identity
- HPLC:
-
High-performance liquid chromatography
- TLC:
-
Thin layer chromatography
- AAI:
-
Average amino acid identity
- POCP:
-
Percentage of conserved proteins
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Acknowledgements
The GenBank accession number for the 16S rRNA gene sequence of strain XSD401T is MN330029. The whole-genome shotgun project of strain XSD401T has been deposited at GenBank under the accession number JAHVXI000000000
Funding
This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).
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Ping, XY., Wang, K., Zhang, JY. et al. Psychroserpens luteolus sp. nov., isolated from Gelidium, reclassification of Ichthyenterobacterium magnum as Psychroserpens magnus comb. nov., Flavihalobacter algicola as Psychroserpens algicola comb. nov., Arcticiflavibacter luteus as Psychroserpens luteus comb. nov. Arch Microbiol 204, 279 (2022). https://doi.org/10.1007/s00203-022-02895-w
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DOI: https://doi.org/10.1007/s00203-022-02895-w