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Pinirhizobacter soli gen. nov., sp. nov., a novel low temperature resistant gammaproteobacterium in the family Rhodanobacteraceae isolated from rhizospheric soil of Larix gmelinii

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Abstract

Three yellow-colored strains, NC2-4-308T, NC3-4-326 and NA3-4-109, were isolated from the rhizosphere soil of Larix gmelinii in Nanwenghe Nature Reserve, Great Khingan, China. These strains were oxidase- and catalase-positive and Gram-staining-negative. The cells were non-motile short rods that were aerobic and non-spore-forming. Growth occurred at pH values of 5.0–8.0 and at 0–4% (w/v) NaCl. The three strains were resistant to low temperature and grew at 2–35 °C. The principal fatty acids (> 5%) were summed feature 9, iso-C15:0, iso-C17:0 and anteiso-C15:0. The predominant quinone was ubiquinone-8. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, two unidentified phospholipids, three unidentified lipids and three unidentified aminophospholipids. The DNA G + C content of the type species was 64.0 mol%. The 16S rRNA gene sequence similarities among the three strains are more than 99.9%, indicating they belong to the same species. Phylogenetic analysis of the 16S rRNA gene, whole-genome sequences, the low ANI (74.2–75.5%) and dDDH (19.3–20.1%) hybridization values enabled differentiation of strains NC2-4-308T, NC3-4-326 and NA3-4-109 from the members of related genera. The combined data from the morphological, physiological, biochemical and chemotaxonomic tests indicate the three strains as a novel genus and a novel species in the family Rhodanobacteraceae. Therefore, we propose a novel genus with the name Pinirhizobacter soli gen. nov., sp. nov., for which the type strain is NC2-4-308T (= CFCC 14693T = KCTC 72394T).

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The datasets used or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This work was funded by the National Infrastructure of Microbial Resources (NIMR-2020-7) and the National Natural Science Foundation of China (Grant NO. 31570603).

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Authors and Affiliations

  1. Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, 100091, People’s Republic of China

    Han Xue, Chun-gen Piao & Yong Li

  2. Institute of Wetland Research, Chinese Academy of Forestry, Beijing, 100091, People’s Republic of China

    Ying-hua Lin

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  1. Han Xue
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  2. Chun-gen Piao
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  3. Ying-hua Lin
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  4. Yong Li
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Correspondence to Yong Li.

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Communicated by Erko Stackebrandt.

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Xue, H., Piao, Cg., Lin, Yh. et al. Pinirhizobacter soli gen. nov., sp. nov., a novel low temperature resistant gammaproteobacterium in the family Rhodanobacteraceae isolated from rhizospheric soil of Larix gmelinii. Arch Microbiol 204, 283 (2022). https://doi.org/10.1007/s00203-022-02867-0

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  • DOI: https://doi.org/10.1007/s00203-022-02867-0

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