Abstract
A Gram-stain-negative, rod-shaped (0.3–0.6 × 0.9–2.2 µm), facultatively aerobic, non-motile and yellow-coloured bacterium, designated strain F6397T, was isolated from a marine sediment in Weihai, PR China. Growth of strain F6397T occurred at 4–37 °C (optimum, 30–33 °C), pH 6.0–9.0 (optimum, 7.5) and in the presence of 1–12% (optimum, 3.0%) (w/v) NaCl. Strain F6397T showed oxidase- and catalase-positive activities. Phylogenetic analyses based on the 16S rRNA gene sequence revealed that the strain was assigned to the genus Winogradskyella. Strain F6397T exhibited 95.5–98.1% sequence similarities to recognized species of the genus Winogradskyella. The average nucleotide identity (ANI) scores with Winogradskyella ludwigii HL116T, Winogradskyella litoriviva KMM 6491 T and Winogradskyella thalassocola KMM 3907 T were 80.1, 78.9 and 82.6%, respectively. The digital DNA–DNA hybridization (dDDH) scores were 23.5, 22.9 and 25.7%, respectively. The genomic DNA G + C content was 33.5 mol%. Strain F6397T contained iso-C15:0, iso-C15:1G, iso-C15:0 3-OH and iso-C17:0 3-OH as the predominant fatty acid and MK-6 as the predominant menaquinone. The polar lipid profiles contained phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. On the basis of the evidence presented in this study, strain F6397T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella marina sp. nov. is proposed. The type strain is F6397T (= KCTC 82422 T = MCCC 1H00438T).
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Data availability
The GenBank accession number for the 16S rRNA gene sequence and Whole Genome Shotgun project of strain F6397T are MW422811 and JADOET000000000, respectively.
Abbreviations
- MCCC:
-
Marine Culture Collection of China
- KCTC:
-
The Korean Collection for Type Cultures
- ANI:
-
Average nucleotide identity
- GGDC:
-
Genome-to-genome distance calculator
- dDDH:
-
Digital DNA–DNA hybridization
- MEGA:
-
Molecular evolutionary genetics analysis
- MIDI:
-
Microbial identification system
- HPLC:
-
High-performance liquid chromatography
- TLC:
-
Thin-layer chromatography
- PG:
-
Phosphatidylglycerol
- PE:
-
Phosphatidylethanolamine
- PC:
-
Phosphatidylcholine
- DPG:
-
Diphosphatidylglycerol
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
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Acknowledgements
The scanning electron microscopy was supported by the Physical Chemical Materials Analytical and Testing Center of Shandong University at Weihai.
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Strain F6397T was isolated by J-SB. Material preparation, experimental operation, data collection and analysis were performed by J-SB, X-ZS and SW. The manuscript was written by J-SB. Project guidance and critical revision of manuscripts was performed by Z-JD. All authors read and approved the final manuscript.
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Communicated by Erko Stackebrandt.
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Bo, J., Song, X., Wang, S. et al. Winogradskyella marina sp. nov., isolated from marine sediment. Arch Microbiol 203, 5381–5386 (2021). https://doi.org/10.1007/s00203-021-02517-x
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DOI: https://doi.org/10.1007/s00203-021-02517-x