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Metallococcus carri gen. nov., sp. nov., a novel member of the family Dermacoccaceae isolated from an automotive air conditioning system

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Abstract

Strain designated DB0510T was isolated from an automobile evaporator core collected in Korea. Cells are gram-stain-positive, aerobic, and coccoid. The strain grew at 15–45 ℃, pH 5.0–8.5 and 0–8.0% (w/v) NaCl. Growth occurs on R2A, trypticase soy agar, Luria–Bertani agar, and nutrient agar. Phylogenetic analysis showed that the strain belongs to the family Dermacoccaceae and strain DB0510T was distinctly separated from validly named genera of this family. Signature nucleotides in 16S rRNA gene sequence revealed that the strain contained the Dermacoccaceae family-specific 16S rRNA signature nucleotides patterns. The major fatty acids were C17:0 and C17:1 cis-9. The only menaquinone was MK-8 (H4). The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, and two unidentified lipids. The diagnostic cell-wall amino acid at position 3 of the peptide subunit was found to be lysine. The cell-wall peptidoglycan contained alanine, aspartic acid, glutamic acid, glycine, lysine, and serine and thus the peptidoglycan type was concluded to be of A4α type with Lys-Gly-Ser-Asp interpeptide bridge. The genome size was 3.49 Mbp and G + C content of the genome DNA was 69.4 mol%. On the basis of the phenotypic, genomic, and chemotaxonomic characteristics, strain DB0510T is considered to represent a novel genus and species within the family Dermacoccaceae, for which the name Metallococcus carri gen. nov., sp. nov. is proposed. The type strain of Metallococcus carri is DB0510T (= KACC 19663 T = NBRC 113349 T).

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Funding

This study was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ0152432020)”, Rural Development Administration, Republic of Korea.

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Author notes

  1. Jong Sung Lim and Hyosun Lee contributed equally to this study.

Authors and Affiliations

  1. Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea

    Jong Sung Lim, Hyosun Lee, Jigwan Son & Jong-Ok Ka

  2. University of Science and Technology, Yuseong-gu, Daejeon, 305-850, Republic of Korea

    Song-Gun Kim

  3. Biological Resource Center/ Korean Collection for Type Culture (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea

    Song-Gun Kim

  4. Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, Republic of Korea

    Dong-Uk Kim

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  1. Jong Sung Lim
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Correspondence to Jong-Ok Ka.

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Communicated by Erko Stackebrandt.

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Lim, J.S., Lee, H., Son, J. et al. Metallococcus carri gen. nov., sp. nov., a novel member of the family Dermacoccaceae isolated from an automotive air conditioning system. Arch Microbiol 203, 4073–4079 (2021). https://doi.org/10.1007/s00203-021-02386-4

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  • DOI: https://doi.org/10.1007/s00203-021-02386-4

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