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Population structure of Venturia inaequalis, a hemibiotrophic fungus, under different host resistance specificities in the Kashmir valley

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Abstract

Venturia inaequalis is a notorious fungal pathogen and show classical gene for gene interaction with its apple host. Neutral markers provide clues about history, evolutionary potential, genetic diversity and population structure of V. inaequalis. The genetic diversity and population structure of fungus indicates that the pathogen is highly diverse with the capacity to breach the scab resistance genes. In the present study, we collected 108 V. inaequalis isolates from three apple cultivars differing in Rvi1 resistance gene. Based on the AMOVA, the variation was mostly distributed among the isolates, providing evidence of non-existence of subpopulation in orchards thus founder population is difficult to arise in Kashmir apple orchards. Pair wise genetic differentiation is less due to regular occurrence of gene flow between the populations residing on different orchard as infected material is transported without stringent quarantine measures. Based on principal coordinate analysis and clustering algorithm as implemented in STRUCTURE, we observed admixture between the two subpopulations, which is quite low, suggesting the existence of pre-zygotic and post-zygotic barriers to gene flow and we cannot rule out the existence of other structures shared by accessions belonging to different varieties. Due to the continuous increase in introduction and monoculture of apple varieties, mixed orchard with different host resistance specificities are more suitable for managing the apple scab in Kashmir valley.

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Acknowledgements

The research was partially funded by Department of Biotechnology, Government of India, New Delhi (Grant No: BT/PR17344/AGII/106/1005/2016) to the corresponding author.

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Author notes

  1. Mushtaq Ahmad

    Present address: Plant Virology and Molecular Plant Pathology Laboratory, Division of Plant Pathology, SKUAST-Kashmir, Shalimar, Srinagar, 190025, India

  2. Asha Nabi

    Present address: Division of Plant Pathology, Faculty of Agriculture, SKUAST-K, Sopore, Wadura, 193201, India

Authors and Affiliations

  1. Plant Virology and Molecular Plant Pathology Laboratory, Division of Plant Pathology, SKUAST-Kashmir, Shalimar, Srinagar, 190025, India

    M. S. Dar, Bilal A. Padder, T. A. Sofi, A. A. Mir & M. D. Shah

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  1. M. S. Dar
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Correspondence to Bilal A. Padder.

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Dar, M.S., Padder, B.A., Ahmad, M. et al. Population structure of Venturia inaequalis, a hemibiotrophic fungus, under different host resistance specificities in the Kashmir valley. Arch Microbiol 202, 2245–2253 (2020). https://doi.org/10.1007/s00203-020-01950-8

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