Abstract
Psylloidea are economically important insects causing serious damage to plants by direct feeding and/or vectoring bacterial pathogens. Results reported here indicate the presence of extracellular bacteria in the spermatheca of egg-laying Trioza alacris females. One phylotype, sharing 99 % identity with the non-phytopathogenic bacterium Erwinia tasmaniensis, was identified regardless of methods applied or insect sampling year and location. This is the first study, achieved by ultrastructural, cultural, and 16S rRNA gene-based analysis, of an insect spermatheca microbiota.
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Acknowledgments
We thank Dr. V. Venturi (ICGEB,Trieste, Italy) for providing us with C. violaceum CV026. This work was partially supported by the Italian Ministry of Education, University and Research (project MIUR-PRIN 2008BPLF7Y) to LM and the University of Siena (project 2264-2013-MD-RICBASE_001) to DM.
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Communicated by Erko Stackebrandt.
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Supplementary Fig. 1
Induction of violacein synthesis in C. violaceum CV026 by Erwinia sp. isolates
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Marchini, D., Ciolfi, S., Gottardo, M. et al. Bacteria of the genus Erwinia found in the spermatheca of the laurel psyllid Trioza alacris . Arch Microbiol 196, 901–905 (2014). https://doi.org/10.1007/s00203-014-1039-2
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DOI: https://doi.org/10.1007/s00203-014-1039-2