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A gene cluster for the synthesis of serotype g-specific polysaccharide antigen in Aggregatibacter actinomycetemcomitans

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Abstract

Aggregatibacter actinomycetemcomitans is an important pathogen related to aggressively progressive periodontal breakdown in adolescents and adults. The species can be divided into six serotypes (a–f) according to their surface carbohydrate antigens. Recently, a new serotype g of A. actinomycetemcomitans was proposed. The aim of the present study was to sequence the gene cluster associated with the biosynthesis of the serotype g-specific polysaccharide antigen and develop serotype-specific primers for PCR assay to identify serotype g strains of A. actinomycetemcomitans. The serotype-specific polysaccharide (SSPS) gene cluster of the NUM-Aa 4039 strain contained 21 genes in 21,842-bp nucleotides. The similarity of the SSPS gene cluster sequence was 96.7 % compared with that of the serotype e strain. Seventeen serotype g genes showed more than 90 % homology both in nucleotide and amino acids to the serotype e strain. Three additional genes with 1,579 bp in NUM-Aa 4039 were inserted into the corresponding ORF13 of the serotype e strain. The serotype g-specific primers were designed from the insertion region of NUM-Aa 4039. Serotypes of the a–f strains were not amplified by serotype-specific g primers; only NUM-Aa 4039 showed an amplicon band. The NUM-Aa 4039 strain was three genes in the SSPS gene cluster different from those of serotype e strain. The specific primers derived from these different regions are useful for identification and distribution of serotype g strain among A. actinomycetemcomitans from clinical samples.

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Authors and Affiliations

  1. Department of Oral Microbiology, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, Matsudo, Chiba, 271-8587, Japan

    Osamu Tsuzukibashi, Masanori Saito, Masatomo Hirasawa & Kazuko Takada

  2. Department of Crown Bridge Prosthodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan

    Taira Kobayashi

  3. Department of Special Needs Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan

    Koji Umezawa

  4. Department of Renascent Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan

    Fumio Nagahama

  5. Department of Genome Medicine, Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan

    Takachika Hiroi

Authors
  1. Osamu Tsuzukibashi
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  2. Masanori Saito
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  3. Taira Kobayashi
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  4. Koji Umezawa
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  5. Fumio Nagahama
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  6. Takachika Hiroi
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  7. Masatomo Hirasawa
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  8. Kazuko Takada
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Corresponding author

Correspondence to Kazuko Takada.

Additional information

Communicated by Erko Stackebrandt.

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Tsuzukibashi, O., Saito, M., Kobayashi, T. et al. A gene cluster for the synthesis of serotype g-specific polysaccharide antigen in Aggregatibacter actinomycetemcomitans . Arch Microbiol 196, 261–265 (2014). https://doi.org/10.1007/s00203-014-0965-3

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  • DOI: https://doi.org/10.1007/s00203-014-0965-3

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