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Pseudomonas oryzae sp. nov. isolated from a paddy soil in South China

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Abstract

A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3T, was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 %), at pH 5.5–9.0 (optimum pH 7.0) and at 25–42 °C (optimum 30–37 °C) in liquid Reasoner’s 2A medium. Analysis of the 16S rRNA gene and gyrB gene sequences revealed that strain WM-3T was most closely related to the type strains of the species Pseudomonas linyingensis and Pseudomonas sagittaria. Its sequence similarities with P. linyingensis CGMCC 1.10701T and P. sagittaria JCM 18195T were 97.4 and 97.3 %, respectively, for 16S rRNA gene, and were 94.1 and 94.2 %, respectively, for gyrB gene. DNA–DNA hybridization between strain WM-3T and these two type strains showed relatedness of 35.6 and 30.9 %, respectively. G+C content of genomic DNA was 69.4 mol%. The whole-cell fatty acids mainly consisted of C16:0 (30.0 %), C16:1 ω6c and/or C16:1 ω7c (19.3 %) and C18:1 ω6c and/or C18:1 ω7c (16.3 %). The results of phenotypic, chemotaxonomic and genotypic analyses clearly indicated that strain WM-3T belongs to genus Pseudomonas but represents a novel species, for which the name Pseudomonas oryzae sp. nov. is proposed. The type strain is WM-3T (=KCTC 32247T =CGMCC 1.12417T).

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Acknowledgments

This work was supported by the Team Project of Guangdong Natural Science Foundation (S2011030002882, S2012030006114 and S2011010002452) and the Science and Technology Planning Project of Guangdong Province, China (2012B010500035 and 2012B030800008).

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Authors and Affiliations

  1. Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou, 510650, People’s Republic of China

    Zhen Yu, Guiqin Yang, Shungui Zhou & Li Zhuang

  2. Chinese Research Academy of Environmental Sciences, Beijing, 100012, People’s Republic of China

    Ming Chang

  3. Chemistry and Materials Institute, Sichuan Normal University, Chengdu, 610068, People’s Republic of China

    Min Wu

Authors
  1. Zhen Yu
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  2. Ming Chang
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  3. Min Wu
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  4. Guiqin Yang
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  5. Shungui Zhou
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  6. Li Zhuang
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Corresponding author

Correspondence to Shungui Zhou.

Additional information

Communicated by Erko Stackebrandt.

Zhen Yu and Ming Chang have contributed equally to this work.

The GenBank accession numbers for the 16S rRNA and gyrB gene sequences of strain WM-3T are KF317694 and KF317695, respectively, and the GenBank accession number for gyrB gene sequence of Pseudomonas linyingensis CGMCC 1.10701T is KF056321.

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Yu, Z., Chang, M., Wu, M. et al. Pseudomonas oryzae sp. nov. isolated from a paddy soil in South China. Arch Microbiol 195, 815–822 (2013). https://doi.org/10.1007/s00203-013-0930-6

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  • DOI: https://doi.org/10.1007/s00203-013-0930-6

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