Abstract
Out of 534 psychrotrophic bacteria, 12 bacteria were selected on the basis of plant growth promoting activities at 4 °C and identified as Pseudomonas genus. These strains showed high level of genetic polymorphisms based on RAPD and rep-PCR fingerprinting. This genetic variability revealed that isolates belonging to same species were as high as the variability among different species. Further inoculation of these Pseudomonas strains significantly improves root/shoot biomass and nutrients uptake of lentil plant as compared to non-bacterized control after 40 days of seed showing. Agglomerative hierarchical clustering analysis of pot assay results revealed that genetically diverse strains showing the same prototype in functional parameter and representing diverse blueprint of plant growth promoting attributes. Results of present findings explain the huge beneficial microbial resources from root zone of hilly crops of Himalayan region that could be effectively exploited as bio-inoculums for cold climatic condition.
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Acknowledgments
The research leading to these results has received funding from the Indian Council of Agricultural Research (ICAR), New Delhi, India, under grant agreement entitled “Application of microorganisms in agriculture and allied sectors (AMAAS).” Our special thanks to Director, Dr. J.C Bhatt, Dr. J.K. Bisht, Head CPD and Dr. Jeevanandan, V.P.K.A.S, Almora, India, for their support and encouragement during study. Mr. Sanjay Kumar, V.P.K.A.S, Almora, India, highly acknowledged for his active support during nutrient analysis.
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Bisht, S.C., Mishra, P.K. & Joshi, G.K. Genetic and functional diversity among root-associated psychrotrophic Pseudomonad’s isolated from the Himalayan plants. Arch Microbiol 195, 605–615 (2013). https://doi.org/10.1007/s00203-013-0908-4
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DOI: https://doi.org/10.1007/s00203-013-0908-4