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Determination of zeta potential in Planctomycetes and its application in heavy metals toxicity assessment

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Abstract

Zeta potential of Planctomycetes was evaluated under different environmental conditions and correlated to cell viability. Phylogenetically distinct strains of the Planctomycetes presented different negative zeta potential values. More negative values were associated with Rhodopirellula spp. and related to the great amount of fimbriae in these species. Milli-Q water was chosen as the best dispersion media to perform the measurements. Zeta potential increased with ionic strength and varied with pH. In the physiological range of pH 5.0–9, zeta potential remained low and Rhodopirellula sp. strain LF2 cells were viable. Out of this range, zeta potential increased significantly and viability decreased. The effect on zeta potential of arsenic, cadmium, chromium, copper, lead, nickel, and zinc was assessed in Rhodopirellula sp. strain LF2. Zeta potential increased with increasing toxicity of the heavy metals in a dose–response way. This result was confirmed by the results observed for Rhodopirellula baltica strain SH1 under copper toxicity. Lead was the most toxic metal and zinc was the least toxic as observed by zeta potential and viability. The results support a correlation between zeta potential and cell viability which seem to indicate the possibility to use it as a viability predictor for the effects of heavy metals toxicity.

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Acknowledgments

The second author was financed by FCT (PhD grant SFRH/BD/35933/2007).

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Authors and Affiliations

  1. CIMAR/CIIMAR, Interdisciplinary Centre for Marine and Environmental Research, University of Porto, Porto, Portugal

    Olga Maria Lage & Joana Bondoso

  2. Department of Biology, Faculty of Sciences, University of Porto, FC4, Rua do Campo Alegre s/n, 4150-069, Porto, Portugal

    Olga Maria Lage & Joana Bondoso

  3. Paralab, SA, Porto, Portugal

    José A. M. Catita

  4. CEBIMED, Faculty of Health Sciences, University Fernando Pessoa, Porto, Portugal

    José A. M. Catita

Authors
  1. Olga Maria Lage
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  2. Joana Bondoso
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  3. José A. M. Catita
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Correspondence to Olga Maria Lage.

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Communicated by Erko Stackebrandt.

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Lage, O.M., Bondoso, J. & Catita, J.A.M. Determination of zeta potential in Planctomycetes and its application in heavy metals toxicity assessment. Arch Microbiol 194, 847–855 (2012). https://doi.org/10.1007/s00203-012-0818-x

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  • DOI: https://doi.org/10.1007/s00203-012-0818-x

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