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A cryptic type I polyketide synthase (cpk) gene cluster in Streptomyces coelicolor A3(2)

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Abstract

The chromosome of Streptomyces coelicolor A3(2), a model organism for the genus Streptomyces, contains a cryptic type I polyketide synthase (PKS) gene cluster which was revealed when the genome was sequenced. The ca. 54-kb cluster contains three large genes, cpkA, cpkB and cpkC, encoding the PKS subunits. In silico analysis showed that the synthase consists of a loading module, five extension modules and a unique reductase as a terminal domain instead of a typical thioesterase. All acyltransferase domains are specific for a malonyl extender, and have a B-type ketoreductase. Tailoring and regulatory genes were also identified within the gene cluster. Surprisingly, some genes show high similarity to primary metabolite genes not commonly identified in any antibiotic biosynthesis cluster. Using western blot analysis with a PKS subunit (CpkC) antibody, CpkC was shown to be expressed in S. coelicolor at transition phase. Disruption of cpkC gave no obvious phenotype.

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Abbreviations

AT:

Acyltransferase

ACP:

Acyl carrier protein

DH:

Dehydratase

DEBS:

6-Deoxyerythronolide synthase

ER:

Enoyl reductase

KR:

Ketoreductase

KS:

β-Ketoacylsynthase

MS:

Mannitol soya flour medium

NRPS:

Non-ribosomal peptide synthetase

PKS:

Polyketide synthase

SMM:

Supplemented minimal medium

TD:

Terminal reductase

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Acknowledgments

This manuscript is dedicated to the late Katarzyna Kuczek. We thank David Hopwood, Wolfgang Wohlleben, Tillman Weber, Jolanta Zakrzewska-Czerwińska and Kira Weissman for critical reading of the manuscript. K. Pawlik, M. Kotowska and K. Kuczek were supported by Polish Ministry of Scientific Research and Information Technology, grant number 3 P04B 004 25 and by the Institute of Immunology and Experimental Therapy. E. Takano was supported from a grant from Human Frontier Science Program RG0330/1998. K. Pawlik was also supported by the John Innes Foundation. Antibodies were raised in the Institute of Immunology and Experimental Therapy in Wroclaw.

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Authors and Affiliations

  1. Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wroclaw, Poland

    Krzysztof Pawlik, Magdalena Kotowska & Katarzyna Kuczek

  2. John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK

    Keith F. Chater & Eriko Takano

  3. Department of Microbial Physiology, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Kerklaan 30, 9751 NN, Haren, The Netherlands

    Eriko Takano

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  1. Krzysztof Pawlik
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  2. Magdalena Kotowska
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  3. Keith F. Chater
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Correspondence to Eriko Takano.

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Pawlik, K., Kotowska, M., Chater, K.F. et al. A cryptic type I polyketide synthase (cpk) gene cluster in Streptomyces coelicolor A3(2). Arch Microbiol 187, 87–99 (2007). https://doi.org/10.1007/s00203-006-0176-7

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  • DOI: https://doi.org/10.1007/s00203-006-0176-7

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