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Production of piscicolin 126 by Carnobacterium maltaromaticum UAL26 is controlled by temperature and induction peptide concentration

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Abstract

Carnobacterium maltaromaticum UAL26 produces the antimicrobial peptides (bacteriocins) piscicolin 126, first isolated from C. maltaromaticum JG126, and carnobacteriocin BM1, first isolated from C. maltaromaticum LV17. C. maltaromaticum UAL26 is especially inhibitory to strains of Listeria monocytogenes. Bacteriocin activity is not observable in the supernatant of cultures of UAL26 grown in liquid media at 25°C, but at temperatures less than 19°C bacteriocin activity can be detected. In contrast to JG126, the piscicolin 126 operon is downregulated in UAL26 at higher temperature, and piscicolin 126 mRNA is not detected when UAL26 is grown at 25°C. Bacteriocin production in UAL26 grown at 15°C can be induced by addition of 10−10 M of chemically synthesized piscicolin 126 induction peptide (PisN). However, induction of bacteriocin production in UAL26 grown at 25°C requires 10−7 M of PisN. The sequence of the piscicolin 126 operon in UAL26 contains 34 single nucleotide differences compared with the piscicolin 126 operon in JG126, including single nucleotide differences in the immunity, histidine kinase, dedicated ABC-transporter and accessory genes, as well as a single nucleotide deletion in the transport accessory gene. This deletion causes a frameshift, resulting in truncation of the PisE transport accessory protein in UAL26.

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Abbreviations

ABC:

ATP binding cassette

LAB:

Lactic acid bacteria

AU:

Arbitrary units

AP:

All-Purpose

APT:

All-Purpose Tween

MALDI-TOF:

Matrix assisted laser desorption/ionization-time-of-flight

DEPC:

Diethyl pyrocarbonate

RP-HPLC:

Reverse-phase HPLC

IP:

Induction peptide

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Acknowledgments

We would like to give special acknowledgement to Denise Rivard and Adam Olson. We gratefully acknowledge the Natural Sciences and Engineering Research Council for providing scholarship assistance and Discovery grant funding, Alberta Ingenuity for a Studentship Award, and the Canada Research Chair in Bioorganic and Medicinal Chemistry and the Advanced Food and Biomaterials Networks of Centres of Excellence for financial support.

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Authors and Affiliations

  1. Department of Agricultural, Food, and Nutritional Science, 4-10 Agriculture Forestry Centre, University of Alberta, Edmonton, AB, Canada, T6G 2P5

    Lucas J. Gursky, Michael E. Stiles & Lynn M. McMullen

  2. Department of Chemistry, University of Alberta, Edmonton, AB, Canada, T6G 2G2

    Nathaniel I. Martin, Darren J. Derksen, Marco J. van Belkum & John C. Vederas

  3. Faculty of Pharmacy and Pharmaceutical Science, University of Alberta, Edmonton, AB, Canada, T6G 2N8

    Kamaljit Kaur

Authors
  1. Lucas J. Gursky
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  2. Nathaniel I. Martin
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  3. Darren J. Derksen
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  4. Marco J. van Belkum
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  5. Kamaljit Kaur
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  6. John C. Vederas
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  7. Michael E. Stiles
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  8. Lynn M. McMullen
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Correspondence to Lynn M. McMullen.

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Gursky, L.J., Martin, N.I., Derksen, D.J. et al. Production of piscicolin 126 by Carnobacterium maltaromaticum UAL26 is controlled by temperature and induction peptide concentration. Arch Microbiol 186, 317–325 (2006). https://doi.org/10.1007/s00203-006-0147-z

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  • DOI: https://doi.org/10.1007/s00203-006-0147-z

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