Abstract
Introduction and hypothesis
Low anesthetic bladder capacity has been shown to be a biomarker for bladder-centric interstitial cystitis/bladder pain syndrome (IC/BPS). The goal of this study was to determine if histopathological evidence from bladder biopsies supports anesthetic bladder capacity (BC) as a marker to distinguish a bladder-centric IC/BPS subtype.
Methods
From a review of our large IC/BPS cohort of patients undergoing hydrodistention, we identified a total of 41 patients with low BC (≤ 400 ml); an additional 41 consecutive patients with BC > 400 ml were selected as the comparator group. The original bladder mucosal biopsy pathology slides were re-reviewed by a single pathologist (blinded to patient information) using a standardized grading scale developed for this study.
Results
Histologically, the low BC subjects exhibited higher levels of acute inflammation (p = 0.0299), chronic inflammation (p = 0.0139), and erosion on microscopy (p = 0.0155); however, there was no significant difference in mast cell count between groups (p = 0.4431). There was no significant gender difference between the groups; female patients were the majority in both groups (low BC: 94.12%, non-low BC: 100%; p = 0.1246). Individuals in the low BC group were older (p < 0.0001), had a higher incidence of Hunner’s lesions on cystoscopy (p < 0.0001), and had significantly higher scores, i.e., more bother symptoms, on two IC/BPS questionnaires (ICPI, p = 0.0154; ICSI, p = 0.0005).
Conclusions
IC/BPS patients with low anesthetic bladder capacity have histological evidence of significantly more acute and chronic inflammation compared with patients with a non-low bladder capacity. These data provide additional evidence to support low bladder capacity as a marker of a distinct bladder-centric IC/BPS phenotype.
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References
Walker SJ, Zambon J, Andersson K-E, et al. Bladder capacity is a biomarker for a bladder centric versus systemic manifestation in interstitial cystitis/bladder pain syndrome. J Urol. 2017;198(2):369–75.
Hanno PM, Erickson D, Moldwin R, Faraday MM. American urological association. Diagnosis and treatment of interstitial cystitis/bladder pain syndrome: AUA guideline amendment. J Urol. 2015;193(5):1545–53.
Berry SH, Elliott MN, Suttorp M, et al. Prevalence of symptoms of bladder pain syndrome/interstitial cystitis among adult females in the United States. J Urol. 2011;186(2):540–4.
Hanno PM, Burks DA, Clemens JQ, et al. AUA guideline for the diagnosis and treatment of interstitial cystitis/bladder pain syndrome. J Urol. 2011;185(6):2162–70.
Barr S. Diagnosis and management of interstitial cystitis. Obstet Gynecol Clin N Am. 2014;41(3):397–407.
Quillin RB, Erickson DR. Management of interstitial cystitis/bladder pain syndrome. Urol Clin North Am. 2012;39(3):389–96.
Hohenfellner M, Black P, Linn JF, Dahms SE, Thüroff JW. Surgical treatment of interstitial cystitis in women. Int Urogynecol J Pelvic Floor Dysfunct. 2000;11(2):113–9.
Colaco M, Koslov DS, Keys T, et al. Correlation of gene expression with bladder capacity in interstitial cystitis/bladder pain syndrome. J Urol. 2014;192(4):1123–9.
Tomaszewski JE, Landis JR, Russack V, et al. Biopsy features are associated with primary symptoms in interstitial cystitis: results from the interstitial cystitis database study. Urology. 2001 Jun 1;57(6):67–81.
O’Leary MP, Sant GR, Fowler FJ, Whitmore KE, Spolarich-Kroll J. The interstitial cystitis symptom index and problem index. Urology. 1997;49(5A Suppl):58–63.
Parsons CL, Dell J, Stanford EJ, et al. Increased prevalence of interstitial cystitis: previously unrecognized urologic and gynecologic cases identified using a new symptom questionnaire and intravesical potassium sensitivity. Urology. 2002;60(4):573–8.
Mazeaud C, Rigaud J, Levesque A, et al. Stratification of patients with interstitial cystitis/bladder pain syndrome according to the anatomical bladder capacity. Urology. 2018.
Buckley M, Walls AF. Identification of mast cells and mast cell subpopulations. Methods in molecular medicine 2008; 285–97.
Walls AF, Amalinei C. Detection of mast cells and basophils by immunohistochemistry. 2014; 117–34.
Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M. Characterization of mast cell populations using different methods for their identification. Histochem Cell Biol. 2017;147(6):683–94.
Sant GR, Kempuraj D, Marchand JE, Theoharides TC. The mast cell in interstitial cystitis: role in pathophysiology and pathogenesis. Urology. 2007;69(4):S34–40.
Kim A, Han J-Y, Ryu C-M, et al. Histopathological characteristics of interstitial cystitis/bladder pain syndrome without Hunner lesion. Histopathology. 2017;71(3):415–24.
Logadottir Y, Delbro D, Lindholm C, Fall M, Peeker R. Inflammation characteristics in bladder pain syndrome ESSIC type 3C/classic interstitial cystitis. Int J Urol. 2014;21:75–8.
Larsen MS, Mortensen S, Nordling J, Horn T. Quantifying mast cells in bladder pain syndrome by immunohistochemical analysis. BJU Int. 2008;102(2):204–7.
Kastrup J, Hald T, Larsen S, Nielsen VG. Histamine content and mast cell count of detrusor muscle in patients with interstitial cystitis and other types of chronic cystitis. Br J Urol. 1983;55(5):495–500.
Patnaik SS, Laganà AS, Vitale SG, et al. Etiology, pathophysiology and biomarkers of interstitial cystitis/painful bladder syndrome. Arch Gynecol Obstet. 2017;295(6):1341–59.
Gamper M, Regauer S, Welter J, Eberhard J, Viereck V. Are mast cells still good biomarkers for bladder pain syndrome/interstitial cystitis? J Urol. 2015;193(6):1994–2000.
Richter B, Hesse U, Hansen AB, et al. Bladder pain syndrome/interstitial cystitis in a Danish population: a study using the 2008 criteria of the European Society for the Study of interstitial cystitis. BJU Int. 2010;105(5):660–7.
Liu H-T, Shie J-H, Chen S-H, Wang Y-S, Kuo H-C. Differences in mast cell infiltration, e-cadherin, and zonula occludens-1 expression between patients with overactive bladder and interstitial cystitis/bladder pain syndrome. Urology. 2012;80(1):225.e13–8.
Hanno P, Levin RM, Monson FC, et al. Diagnosis of interstitial cystitis. J Urol. 1990;143(2):278–81.
Akiyama Y, Homma Y, Maeda D. Pathology and terminology of interstitial cystitis/bladder pain syndrome: a review. Histol Histopathol 2018;18028.
Akiyama Y, Maeda D, Morikawa T, et al. Digital quantitative analysis of mast cell infiltration in interstitial cystitis. Neurourol Urodyn. 2018;37(2):650–7.
Acknowledgements
The authors thank the study participants. Funding for this study was provided, in part, by the Interstitial Cystitis Association (2014 Pilot Research Program Grant Award; SJW) and the NIDDK (R21DK106554-01; SJW).
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Schachar, J.S., Evans, R.J., Parks, G.E. et al. Histological evidence supports low anesthetic bladder capacity as a marker of a bladder-centric disease subtype in interstitial cystitis/bladder pain syndrome. Int Urogynecol J 30, 1863–1870 (2019). https://doi.org/10.1007/s00192-019-04038-0
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DOI: https://doi.org/10.1007/s00192-019-04038-0