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TGF-beta 1 is a potential regulator of vaginal tropoelastin production

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Abstract

Introduction and hypothesis

Our aims were to correlate transforming growth factor (TGF)-β1 and elastin mRNA expression in the vagina of women and to measure the effects of TGF-β1 on vaginal smooth muscle cell (SMC) proliferation and tropoelastin production.

Methods

Vaginal walls were sampled in women (n = 20). TGF-β1 and elastin mRNA expression was assessed by RT-PCR. SMC cultures were performed from vaginal wall biopsies. SMC were incubated with TGF-β1, and cell proliferation was assessed by MTT-assay. Tropoelastin production was measured by the Fastin Elastin Assay.

Results

There was a significant positive correlation between TGF-β1 and elastin mRNA (r = 0.784, P < 0.01). SMC proliferation was significantly increased by 10 ng/mL TGF-β1 [relative cell number, mean ± SD, 198% ± 32% of control (P = 0.01)]. Tropoelastin production was significantly increased by TGF-β1 [mean ± SD, 645% ± 180% of control (P = 0.01)].

Conclusions

There is a positive correlation between TGF-β1 and elastin mRNA expression in the vaginal wall. In vitro, TGF-β1 increases vaginal tropoelastin production in vaginal SMC.

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Authors and Affiliations

  1. Division of Female Pelvic Medicine and Reconstructive Surgery, Department of Obstetrics and Gynecology, University of Miami, Miller School of Medicine, Jackson Memorial Hospital, P.O. Box 016960, Miami, FL, 33010, USA

    Peter Takacs, Sujata Yavagal, Nahida Chakhtoura & Carlos A. Medina

  2. Department of Anesthesiology, University of Miami, Miller School of Medicine, Miami, FL, USA

    Yanping Zhang & Keith Candiotti

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  1. Peter Takacs
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  2. Yanping Zhang
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  3. Sujata Yavagal
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  4. Keith Candiotti
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  5. Nahida Chakhtoura
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  6. Carlos A. Medina
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Correspondence to Peter Takacs.

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Takacs, P., Zhang, Y., Yavagal, S. et al. TGF-beta 1 is a potential regulator of vaginal tropoelastin production. Int Urogynecol J 23, 357–363 (2012). https://doi.org/10.1007/s00192-011-1589-7

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  • DOI: https://doi.org/10.1007/s00192-011-1589-7

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