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Mobilization of bone marrow-derived mesenchymal stem cells into the injured tissues after intraarticular injection and their contribution to tissue regeneration

  • Experimental Study
  • Published:
Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

The purpose of present study was to evaluate active mobilization effect of mesenchymal stem cells (MSCs) into injured tissues after intraarticular injection of MSCs, and to evaluate their contribution to tissue regeneration. MSCs, which were obtained from green fluorescent protein (GFP) transgenic Sprague–Dawley (SD) rat and cultivated, were injected into normal SD rats in which multiple tissues had been injured including anterior cruciate ligament (ACL), medial meniscus, and articular cartilage of the femoral condyles. At 4 weeks after injection of MSCs, fluorescent microscopic observation, immunohistochemical or histological examinations were performed to evaluate mobilization of MSCs into injured tissue and their contribution to tissue regeneration. In the group of 1 × 106 MSCs injection, GFP positive cells could mobilize into the injured ACL alone in all 8 knees. In the group of 1 × 107 MSCs injection, GFP positive cells were observed in the injured site of ACL in all 8 knees and in the injured site of medial meniscus and cartilage of femoral condyles in 6 of 8 knees. More interestingly, extracellular matrix stained by toluidine blue was present around GFP positive cells in the injured femoral condyles cartilage and medial meniscus, indicating tissue regeneration. Intraarticularly injected MSCs could mobilize into the injured tissues, and probably contributed to tissue regeneration. This study demonstrated the possibility of intraarticular injection of MSCs for the treatment of intraarticular tissue injuries including ACL, meniscus, or cartilage. If this treatment option is established, it can be minimally invasive compared to conventional surgeries for these tissues.

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Acknowledgments

We thank Dr Tomohiro Nakano of Hiroshima University, for their technical assistance with histological analysis and encouragement. The present study was supported in part by Grants-in-Aid for Scientific Research (No. 16209045) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The reported experiments comply with the current laws of Japan.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-Ku, Hiroshima, 734-8551, Japan

    Muhammad Agung, Mitsuo Ochi, Shinobu Yanada, Nobuo Adachi, Yasunori Izuta & Takuma Yamasaki

  2. Hiroshima Prefectural Rehabilitation Center, Hiroshima, Japan

    Katsuhiro Toda

Authors
  1. Muhammad Agung
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  2. Mitsuo Ochi
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  3. Shinobu Yanada
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  4. Nobuo Adachi
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  5. Yasunori Izuta
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  6. Takuma Yamasaki
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  7. Katsuhiro Toda
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Corresponding author

Correspondence to Nobuo Adachi.

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Agung, M., Ochi, M., Yanada, S. et al. Mobilization of bone marrow-derived mesenchymal stem cells into the injured tissues after intraarticular injection and their contribution to tissue regeneration. Knee Surg Sports Traumatol Arthrosc 14, 1307–1314 (2006). https://doi.org/10.1007/s00167-006-0124-8

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  • DOI: https://doi.org/10.1007/s00167-006-0124-8

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