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Transformation of 17β-Estradiol by Phanerochaete chrysosporium in Different Culture Media

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Abstract

The removal of 17β-estradiol (E2) by white-rot fungus Phanerochaete chrysosporium cultured in classic Kirk or potato medium was systematically investigated. Results demonstrated that E2 can be efficiently removed regardless of culture media type. However, the reaction intermediates and transformation pathways varied in different media. Estrone (E1) and estriol (E3) were sequentially generated as intermediates in the potato medium, but these intermediates were absent in Kirk medium. Such results were found to correlate to the peroxidases produced in Kirk medium. These enzymes catalyzed one-electron oxidation of E2 to form radicals that can undergo oxidative coupling. Similar enzymes were not detected in the potato medium, thus E2 underwent in vitro oxidation to form E1 and E3 sequentially. Adding glucose to the potato medium further accelerated such processes. The findings in this study provide insights into estrogen reactions mediated by P. chrysosporium and for potential development of biodegradation methods to reduce estrogen contamination levels.

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Acknowledgments

This study was supported in part by HATCH funds, Northwest A&F University Fundamental Research Funds (QN2013072) and PhD research starting foundation (2013BSJJ118). LZ acknowledges the China Scholarship Council for supporting her visiting study at the University of Georgia, Griffin Campus.

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Authors and Affiliations

  1. College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Lina Zhou

  2. Department of Crop and Soil Sciences, University of Georgia, Griffin, GA, 30223, USA

    Qi Luo & Qingguo Huang

  3. College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing, 210095, China

    Junhe Lu

Authors
  1. Lina Zhou
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  2. Qi Luo
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  3. Junhe Lu
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  4. Qingguo Huang
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Correspondence to Qingguo Huang.

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Zhou, L., Luo, Q., Lu, J. et al. Transformation of 17β-Estradiol by Phanerochaete chrysosporium in Different Culture Media. Bull Environ Contam Toxicol 95, 265–271 (2015). https://doi.org/10.1007/s00128-015-1557-x

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  • DOI: https://doi.org/10.1007/s00128-015-1557-x

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