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Induction of Oxidative Stress and Related Transcriptional Effects of Sodium Fluoride in Female Zebrafish Liver

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Abstract

The effects of sodium fluoride (NaF) exposure on the induction of oxidative stress and alteration of gene expressions were studied in the liver of female zebrafish (Danio rerio). Zebrafish, exposed to 15 ppm NaF for 30 and 90 days, exhibited liver histopathology including hyperplassia, cytoplasmic degeneration and nuclear fragmentation. Antioxidant enzyme (GST, CAT, SOD) activities in the liver altered significantly; the mRNA levels for the genes encoding antioxidant proteins, such as Gst, Cat, Cu/ZnSod, MnSod as well as Gpx were significantly upregulated at 30 days NaF-treatment along with the stress marker gene Hsp70 and phase I detoxyfying gene Cyp1A1. Moreover, the transcriptional pattern of Ucp2, related to mitochondrial reactive oxygen species (ROS) production, upregulated significantly at 90 days NaF-treatment. ROS generation was evidensed by fluoroscence microscopy. The results of this study will help to understand the mechanism of oxidative stress induced by NaF in fish.

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Acknowledgments

Authors acknowledge Dr. Dipak K Mondal for his help in analyzing the histological change and Department of Biotechnology, Govt. of India (DBT Project No. 54/NE/TBP/2010) to AC, University Grants Commission (UGC), New Delhi for the Centre for Advanced Studies (CAS) grant to the Department of Zoology. Authors are also thankful to Dr. Jolly Basak, Department of Biotechnology for carrying out the Real Time PCR. DM is grateful for a DBT Project Fellowship.

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The authors declare that there is no conflict of interest.

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  1. Molecular Genetics Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati, Santiniketan, 731 235, India

    Debdip Mukhopadhyay & Ansuman Chattopadhyay

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  1. Debdip Mukhopadhyay
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  2. Ansuman Chattopadhyay
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Correspondence to Ansuman Chattopadhyay.

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Mukhopadhyay, D., Chattopadhyay, A. Induction of Oxidative Stress and Related Transcriptional Effects of Sodium Fluoride in Female Zebrafish Liver. Bull Environ Contam Toxicol 93, 64–70 (2014). https://doi.org/10.1007/s00128-014-1271-0

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