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Sub-Chronic Toxicity of Senilia senilis Hepatopancreas in Albino Wistar Mice

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Abstract

This study investigates the sub-chronic effects of shellfish possibly contaminated by algal toxins in albino Wistar mice. Following in-feed treatment with hepatopancreas, there were significant (p < 0.05) differences in packed cell volume, haemoglobin concentration, red blood cell count, lymphocyte count and alanine aminotransferase between the different treatment groups and control. Post mortem revealed inflammation and congestion in the gastrointestinal tract of the middle and highest dose groups. The stomach of the mice fed on the highest dose of the hepatopancreas showed severe ulcerations, leaving behind deep cavities and necrotic tissues on the glandular epithelium of the mucosal wall.

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References

  • Amzil Z, Quilliam MA, Hu T, Wright JLC (1999) Winter accumulation of paralytic shellfish toxins in digestive glands of mussels from Arcachon and Toulon (France) without detectable toxic plankton species revealed by interference in the mouse bioassay for lipophilic toxins. Nat Toxins 7:271–277

    Article  CAS  Google Scholar 

  • Cordier S, Monfort C, Miossec L, Richardson S, Belin C (2000) Ecological analysis of digestive cancer mortality related to contamination by diarrhetic shellfish poisoning toxins along the coasts of France. Environ Res Section A 84:145–150

    Article  CAS  Google Scholar 

  • Dorner JL, Hoffman WE, Fillipov MM (1983) Effect of in vitro hemolysis on values for certain porcine serum constituents. Vet Clin Pathol 12:15–19

    Article  CAS  Google Scholar 

  • Fujiki H, Suganuma M, Yoshizawa S, Nishiwaki S, Winyar B, Sugimura T (1991) Mechanisms of action of okadaic acid class tumor promoters on mouse skin. Environ Hlth Perspect 93:211–214

    Article  CAS  Google Scholar 

  • Hu T, Marr J, De Freitas ASW, Quilliam MA, Wright JLC, Pleasance S (1992) New diol esters (of okadaic acid) isolated from cultures of the dinoflagellates P. lima and P. concavum. J Nat Prod 55:1631–1637

    Article  CAS  Google Scholar 

  • Igboeli OO, Asuzu IU (2010) ‘Diarrhetic’ Type Shellfish Poisoning in Nigeria. Bull Environ Contam Toxicol 84:15–18

    Article  CAS  Google Scholar 

  • Ninčević-Gladan Ž, Skejić S, Bužančić M, Marasović I, Arapov J, Ujević I, Bojanić N, Grbec B, Kušpilić G, Vidjak O (2008) Seasonal variability in Dinophysis spp. abundances and diarrhetic shellfish poisoning outbreaks along the eastern Adriatic coast. Botanica Marina 51:449–463

    Article  Google Scholar 

  • Ramstad H, Hovgaard P, Yasumoto T, Larsen S, Aune T (2001) Monthly variations in diarrhetic toxins and yessotoxin in shellfish from coast to the inner parts of the Sognefjord, Norway. Toxicon 39:1035–1043

    Article  CAS  Google Scholar 

  • Stabell OB, Steffenak I, Pedersen K, Underdal B (1991) Diversity of shellfish toxins of “diarrhetic” type revealed by biological and chemical assays. J Toxicol Environ Hlth 33:273–282

    Article  CAS  Google Scholar 

  • Stobo LA, Lacaze J-PCL, Scott AC, Petrie J, Turrell EA (2008) Surveillance of algal toxins in shellfish from Scottish waters. Toxicon 51:635–648

    Article  CAS  Google Scholar 

  • Suganuma M, Suttajit M, Suguri H, Ojika M, Yamada K, Fujiki H (1989) Specific binding of okadaic acid, a new tumor promoter in the mouse skin. FEBS Letters 250:615–618

    Article  CAS  Google Scholar 

  • Vale P, Sampayo MAM (2002) First confirmation of human diarrhoeic poisonings by okadaic acid esters after ingestion of razor clams (Solen marginatus) and green crabs (Carcinus maenas) in Aveiro lagoon, Portugal and detection of okadaic acid esters in phytoplankton. Toxicon 40:989–996

    Article  CAS  Google Scholar 

  • Yasumoto T, Oshima Y, Yanaguchi M (1978) Occurrence of a new type of shellfish poisoning in the Tohoku district. Bull Jpn Soc Fish 44:1249–1255

    Google Scholar 

  • Yasumoto T, Murata M, Oshima Y, Sano M, Matsumoto GK, Clardy J (1985) Diarrhetic shellfish toxins. Tetrahedron 41:1019–1025

    Article  CAS  Google Scholar 

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Acknowledgments

The authors wish to thank Peter Okoye of the Histopathology Unit, University of Nigeria Teaching Hospital, Enugu; Austin Ngene, Medicine Laboratory, Department of Veterinary Medicine and Prof. J.A.O. Okoye, Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka for their technical assistance. We are grateful to Drs. John Burka and Collins Kamunde, Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Canada for their corrections of the manuscript. Funding was from the Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka and the Samuel N.C. Igboeli family. We are grateful to both sources of funds.

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Correspondence to Okechukwu O. Igboeli.

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Igboeli, O.O., Asuzu, I.U. Sub-Chronic Toxicity of Senilia senilis Hepatopancreas in Albino Wistar Mice. Bull Environ Contam Toxicol 84, 657–661 (2010). https://doi.org/10.1007/s00128-010-0022-0

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  • DOI: https://doi.org/10.1007/s00128-010-0022-0

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