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Quantification of Nitroimidazoles Residues in Swine Liver by Liquid Chromatography–Mass Spectrometry with Atmospheric Pressure Chemical Ionization

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Abstract

A liquid chromatography–mass spectrometry method was developed for the simultaneous determination of ronidazole (RNZ), metronidazole (MNZ) and dimetridazole (DMZ) residues in swine liver. Following liquid–liquid extraction, the HLB solid-phase extraction was used for further purification. The targets were detected by atmospheric pressure chemical ionization (APCI) following the reverse phase liquid chromatography separation. Consequently, the detection limits for the method were 0.5 μg/kg for MNZ, 1.0 μg/kg for RNZ and 0.5 μg/kg for DMZ, respectively. The accuracies were determined using swine liver samples fortified at levels of 0.5, 1, 2, and 4 μg/kg and the mean recoveries of the analytes were between 66% and 81%.

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Acknowledgments

Funding supports from Natural Science Foundation of China (20621703) and the Foundation of Ministry of Science & Technology of China (2002BA906A75) are gratefully acknowledged.

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Authors and Affiliations

  1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Shuangqing Street, Haidian District, Beijing, 100085, People’s Republic of China

    Hai Wang & Zijian Wang

  2. Quality Control and Inspection Center for Animal Products, Ministry of Agriculture, China Animal Disease Control Center, Maizidian Street, Chaoyang District, Beijing, 100026, People’s Republic of China

    Hai Wang, SuYing Liu, Yanbing Jiang, Yinliang Wu & Jihao Shan

Authors
  1. Hai Wang
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  2. Zijian Wang
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  3. SuYing Liu
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  4. Yanbing Jiang
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  5. Yinliang Wu
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  6. Jihao Shan
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Correspondence to Hai Wang.

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Wang, H., Wang, Z., Liu, S. et al. Quantification of Nitroimidazoles Residues in Swine Liver by Liquid Chromatography–Mass Spectrometry with Atmospheric Pressure Chemical Ionization. Bull Environ Contam Toxicol 82, 411–414 (2009). https://doi.org/10.1007/s00128-008-9591-6

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  • DOI: https://doi.org/10.1007/s00128-008-9591-6

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