Skip to main content
SpringerLink
Log in

QTL analysis of pest resistance in the wild tomato Lycopersicon pennellii: QTLs controlling acylsugar level and composition

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Some accessions of Lycopersicon pennellii, a wild relative of the tomato Lycopersicon esculentum, are resistant to a number of important pests of cultivated tomato due to the accumulation of acylsugars, which constitute 90% of the exudate of type-IV trichomes in L. pennellii LA716. An interspecific F2 population, created by the cross L. esculentum x L. pennellii LA 716, was surveyed for acylsugar accumulation and subjected to RFLP/QTL analysis to determine the genomic regions associated with the accumulation of acylglucoses, acylsucroses, and total acylsugars, as well as with acylglucoses as a percentage of total acylsugars (mole percent acylglucoses). Data were analyzed using MAPMAKER/QTL with and without a log10 transformation. A threshold value of 2.4 (default value for MAPMAKER/QTL) was used, as well as 95% empirically derived threshold values. Five genomic regions, two on chromosome 2 and one each on chromosomes 3, 4 and 11, were detected as being associated with one or more aspects of acylsugar production. The L. esculentum allele is partially dominant to the L. pennellii allele in the regions on chromosomes 2 and 11, but the L. pennellii allele is dominant in the region on chromosome 3. Throughout this study, we report the comparative effects of analytical methodology on the identification of acylsugar QTLs. Similarities between our results and published results for the genus Solanum are also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bonierbale MW, Plaisted RL, Pineda O, Tanksley SD (1994) QTL analysis of trichome-mediated insect resistance in potato. Theor Appl Gen 87:973–987

    Google Scholar 

  • Buta GJ, Lusby WR, Neal JW Jr., Waters RM, Pittarelli GW (1993) Sucrose esters from Nicotiana gossei active against the greenhouse whitefly Trialeuroides vaporarium. Phytochemistry 32:859–864

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    CAS  PubMed  Google Scholar 

  • Darvasi A, Weinreb A, Minke V, Weller J, Soller M (1993) Detecting marker QTL linkage and estimating QTL gene effect and map location using a saturated genetic map. Genetics 134:943–951

    Google Scholar 

  • Doerge RW (1993) Statistical methods for locating quantitative trait loci with molecular markers. PhD dissertation. North Carolina State University,

  • Doerge RW, Churchill GA (1994) Analysis of molecular marker data. Issues in genetic mapping of quantitative trait loci. In: Proc ASHA/CSSA 2nd Joint Plant Breed Symp. Corvallis, Oregon, pp 27–32

  • Doyle JJ, Dickson EE (1987) Preservation of plant samples for DNA restriction endonuclease analysis. Taxon 36:715–722

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:6–13

    Google Scholar 

  • Feinberg AP, Vogelstein B (1984) Addendum: a technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267

    CAS  PubMed  Google Scholar 

  • Fobes JF, Mudd JB, Marsden MPF (1985) Epicuticular lipid accumulation on the leaves of Lycopersicon pennellii (Corr.) D'Arcy and Lycopersicon esculentum Mill. Plant Physiol 77:567–570

    Google Scholar 

  • Gentile AG, Stoner AK (1968) Resistance in Lycopersicon and Solarium species to the potato aphid. J Econ Entomal 61:1152–1154

    Google Scholar 

  • Gentile AG, Webb RE, Stoner AK (1968) Resistance in Lycopersicon and Solanum to greenhouse whiteflies. J Econ Entomol 61:1355–1357

    Google Scholar 

  • Gentile AG, Webb RE, Stoner AK (1969) Lycopersicon and Solanum spp. resistant to the carmine and two-spotted spider mite. J Econ Entomol 62:834–836

    Google Scholar 

  • Goffreda JC, Ave DA, Mutschler MA, Steffens JC, Tingey WM (1989) Aphid deterrence by glucose esters in the glandular trichome exudate of the wild tomato, L. pennellii. J Chem Ecol 15:2135–2147

    Google Scholar 

  • Goffreda JC, Mutschler MA, Steffens JC (1990) Association of epicuticular sugars with aphid resistance in hybrids with wild tomato. J Am Soc Hort Sci 117:161–164

    Google Scholar 

  • Haldane JBS (1919) The combination of linkage values and the calculation of distance between the loci of linked factors. J Genet 8:299–309

    Google Scholar 

  • Hartigan JA (1985) A failure of likelihood asymptotics for normal distributions. In: LeCam LM, Olsen RA (eds) Proc Berkeley Conf, vol. II pp 807–810

  • Hawthorne DM, Shapiro JA, Tingey WM, Mutschler MA (1992) Trichome-borne and artificially applied acylsugars of wild tomato deter feeding and oviposition of the leafminer, Liriomyza trifolii Entomol Exp Appl 65:65–73

    Google Scholar 

  • Juvik JA, Shapiro JA, Young TE, Mutschler MA (1993) Acylglucoses of the wild tomato Lycopersicon pennellii alter behavior and reduce growth and survival of Helicoverpa zea and Spodoptera exigua. J Econ Entomol 87:482–492

    Google Scholar 

  • Kennedy BS, Nielsen MT, Severson RF, Sisson VA, Stephenson MK, Jackson DM (1992) Leaf surface chemicals from Nicotiana affecting germination of Peronospora tabacina sporangia. J Chem Ecol 18, 1467–1479

    Google Scholar 

  • King RR, Calhoun LA (1988) 2, 3-di-O- and 1, 2, 3-tri-O-acylated glucose esters from the glandular trichomes of Datura metel. Phytochemistry 27:3761–3765

    Google Scholar 

  • King RR, Calhoun LA, Singh RP (1988) 3, 4-di-O and 2,3,4-tri-O-acylated glucose esters from the glandular trichomes of nontuberous Solanum species. Phytochemistry 27:3765–3768

    Google Scholar 

  • King RR, Calhoun LA, Singh RP, Boucher A (1990) Sucrose esters associated with glandular trichomes of wild Lycopersicon species. Phytochemistry 29:2115–2118

    Google Scholar 

  • Kinzer S, Schwager S, Mutschler MA (1990) Mapping of ripening-related or -specific cDNA clones of tomato (Lycopersicon esculentum). Theor Appl Genet 79:489–496

    Google Scholar 

  • Lander E, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199

    CAS  PubMed  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly M, Lincoln S, Newburg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Lemke CA, Mutschler MA (1984) Inheritance of glandular trichomes in crosses between Lycopersicon esculentum and L. pennellii. J Am Soc Hort Sci 109:592–596

    Google Scholar 

  • Liedl BE, Lawson DM, White KK, Shapiro JA, Cohen DE, Carson WG, Trumble JT, Mutschler MA (1995) Acylglucoses of the wild tomato Lycopersicon pennellii (Corr.) D'Arcy alters settling and reduces oviposition of Bemisia argentifulii (Homoptera: Aleyrodidae) J Econ Entomol 88:742–748

    Google Scholar 

  • Lincoln S, Lander E (1992) Systematic detection of errors in genetic linkage data. Genomics 14:604–610

    Google Scholar 

  • Lincoln SE, Daly MJ, Lander ES (1992a) Mapping genes controlling quantitative traits with MAPMAKER/QTL 1.1. Whitehead Institute Technical Report. 2nd edn.

  • Lincoln SE, Daly MJ, Lander ES (1992b) Constructing genetic linkage maps with MAPMAKER/EXP version 3.0: a tutorial and reference manual. Research Technical Report 3rd edn.

  • Liu S-C (1994) Nature and genetic control of hybrid breakdown and segregation distortion in the interspecific F2 populations from the cross Lycopersicon esculentum x L. pennellii. PhD dissertation, Cornell Univ Ithaca, New York

    Google Scholar 

  • Liu S-C, Liedl BE, Mutschler MA (1995) Alterations of the manifestations of hybrid breakdown in Lycopersicon esculentum x L. pennellii F2 populations containing L. esculentum versus L. pennellii cytoplasm. Sexual Plant Reproduction 8:361–368

    Article  CAS  PubMed  Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

    Google Scholar 

  • Minitab (1991) MINITAB. Minitab, Inc. State College, PA

  • Mutschler MA (1990) Transfer of Lycopersicon pennellii cytoplasm into tomato (L. esculentum) does not create cytoplasmic male sterility. Rep Tomato Genet Coop 40:25–26

    Google Scholar 

  • Mutschler MA, Liedl B (1994) Interspecific crossing barriers in Lycopersicon and their relationship to self-incompatibility. In: Williams EG, Clarke AE (eds.) Genetic control of selfincompatibility and reproductive development in flowering plants. Kluwer Academic Publishers, Dordrecht, the Netherlands, pp 164–188

    Google Scholar 

  • Neal JJ, Tingey WM, Steffens JS (1990) Sucrose esters of carboxylic acids in glandular trichomes of Solanum bertaultii deter settling and probing by green peach aphid. J Chem Ecol 16:487–497

    Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Article  CAS  PubMed  Google Scholar 

  • Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations, and environments. Genetics 127:181–197

    Google Scholar 

  • Rodriguez AE, Tingey WM, Mutschler MA (1993) Acylsugars produced by type-IV trichomes of Lycopersicon pennellii deter settling of the green peach aphid, Myzus persicae. J Econ Entomol 86:34–39

    Google Scholar 

  • Schumacher JN (1970) The isolation of 6-O-acetyl-2,3,4-tri-O-[(+)-3-methylvaleryl]-D-glucopyranose from tobacco. Carbohyd. Res 13:1–8

    Google Scholar 

  • Self SG, Liang K (1987) Asymptotic properties of maximum-likelihood estimators and likelihood ratio tests under non-standard conditions. J Am Stat Assoc 82:605–610

    Google Scholar 

  • Severson RF, Johnson AW, Jackson DM (1985) Cuticular constituents of tobacco: factors affecting their production and their role in insect and disease resistance and smoke quality. Recent Adv Tobacco Sci 11:105–174

    Google Scholar 

  • Shapiro JA, Steffens JC, Mutschler MA (1994) Acylsugars of the wild tomato Lycopersicon pennellii in relation to its geographic distribution. J Biochem Syst 22:545–561

    Google Scholar 

  • Tanksley SD (1993) Mapping polygenes. Annu Rev Genetics 27:205–233

    Google Scholar 

  • Tanksley SD, Mutschler MA (1990) Linkage maps of the tomato Lycopersicon esculentum (2n=24). In: Genetics maps (5th edn). Obrien J (ed) Cold Spring Habor Laboratory Press, Cold Spring Harbor, New York, pp 3–15

    Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, DeVincente MC, Bonierbale MW, Broun P, Fulton TM, Giovanoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineida O, Roder M, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes: biological inferences and practical applications. Genetics 132:1141–1160

    CAS  PubMed  Google Scholar 

  • Titterington DM, Smith AFM, Makov UE (1985) Statistical analysis of finite mixture distributions. John Wiley and Sons, N.Y.

    Google Scholar 

  • Young ND, Tanksley SD (1989) Restriction fragment length polymorphism maps and the concept of graphical genotypes. Theor Appl Genet 77:95–101

    Google Scholar 

  • Zamir D, Tadmor Y (1986) Unequal segregation of nuclear genes in plants. Bot Gaz 147:355–358

    Article  Google Scholar 

Download references

Author information

Author notes

  1. R. W. Doerge

    Present address: Statistics and Agronomy Depts, Purdue University, 47907-1399, West Lafayette, IN, USA

  2. S. C. Liu

    Present address: , 3511 Headway Drive, 77082, Houston, TX, USA

  3. B. E. Liedl

    Present address: Biology Department, Central College, 50219, Pella, IA, USA

  4. J. A. Shapiro

    Present address: BHN Research, 16750 Bonita Beach Road, F1 33923, Bonita Springs, USA

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853, Ithaca, N.Y., USA

    M. A. Mutschler, R. W. Doerge, S. C. Liu, J. P. Kuai, B. E. Liedl & J. A. Shapiro

Authors
  1. M. A. Mutschler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. W. Doerge
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. C. Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. P. Kuai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. E. Liedl
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. A. Shapiro
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Salamini

R. W. Doerge · S.-C. Liu · J. P. Kuai contributed equally to the paper, and we ordered randomly

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mutschler, M.A., Doerge, R.W., Liu, S.C. et al. QTL analysis of pest resistance in the wild tomato Lycopersicon pennellii: QTLs controlling acylsugar level and composition. Theoret. Appl. Genetics 92, 709–718 (1996). https://doi.org/10.1007/BF00226093

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00226093

Key words

Search

Navigation