Abstract
Genetic mapping and the selection of closely linked molecular markers for important agronomic traits require efficient, large-scale genotyping methods. A semi-automated multifluorophore technique was applied for genotyping AFLP marker loci in barley and wheat. In comparison to conventional 33P-based AFLP analysis the technique showed a higher resolution of amplicons, thus increasing the number of distinguishable fragments. Automated sizing of the same fragment in different lanes or different gels showed high conformity, allowing subsequent unambigous allele-typing. Simultaneous electrophoresis of different AFLP samples in one lane (multimixing), as well as simultaneous amplification of AFLP fragments with different primer combinations in one reaction (multiplexing), displayed consistent results with respect to fragment number, polymorphic peaks and correct size-calling. The accuracy of semi-automated co-dominant analysis for hemizygous AFLP markers in an F2 population was too low, proposing the use of dominant allele-typing defaults. Nevertheless, the efficiency of genetic mapping, especially of complex plant genomes, will be accelerated by combining the presented genotyping procedures.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 10 April 1999 / Accepted: 11 May 1999
Rights and permissions
About this article
Cite this article
Schwarz, G., Herz, M., Huang, X. et al. Application of fluorescence-based semi-automated AFLP analysis in barley and wheat. Theor Appl Genet 100, 545–551 (2000). https://doi.org/10.1007/s001220050071
Issue Date:
DOI: https://doi.org/10.1007/s001220050071