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Fine mapping of a powdery mildew resistance gene MlIW39 derived from wild emmer wheat (Triticum turgidum ssp. dicoccoides)

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Powdery mildew resistance gene MlIW39, originated from wild emmer wheat accession IW39, was mapped to a 460.3 kb genomic interval on wheat chromosome arm 2BS.

Abstract

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is destructive disease and a significant threat to wheat production globally. The most effective way to control this disease is genetic resistance. However, when resistance genes become widely deployed in agriculture, their effectiveness is compromised by virulent variants that were previously minor components of the pathogen population or that arise from mutation. This necessitates continual search for new sources of resistance in both wheat and its near relatives. In this study, we produced a common wheat line 8D49 (87-1/IW39//2*87-1), which has all-stage immunity to Bgt isolate E09 and many other Chinese Bgt isolates, by transferring powdery mildew resistance from Israeli wild emmer wheat (WEW) accession IW39 to the susceptible common wheat line 87-1. Genetic analysis indicated that the powdery mildew resistance in 8D49 was controlled by a single dominant gene, temporarily designated MlIW39. Genetic linkage analyses with molecular markers showed that MlIW39 was located in a 0.7 cm genetic region between markers QB-3-16 and 7Seq546 on the short arm of chromosome 2B. Fine mapping using three large F2 populations delimited MlIW39 to a physical interval of approximately 460.3 kb region in the WEW reference genome (Zavitan v1.0) that contained six annotated protein-coding genes, four of which had gene structures similar to known disease resistance genes. This provides a foundation for map-based cloning of MlIW39. Markers 7Seq622 and 7Seq727 co-segregating with MlIW39 can be utilized for marker-assisted selection in further genetic studies and wheat breeding.

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Acknowledgements

This work was financially supported by the National Key Research and Development Project of China (2016YFD0101802) and National Natural Science Foundation of China (31671676, 31872865). We thank Dr. R. A. McIntosh for suggestions regarding the manuscript. We are grateful to the referees for their valuable suggestions and comments to improve the manuscript.

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  1. State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China

    Lina Qiu, Nannan Liu, Huifang Wang, Feng Li, Qiang Zhang, Weidong Wang, Weilong Guo, Zhaorong Hu, Jun Ma, Qixin Sun & Chaojie Xie

  2. National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Xiaohan Shi & Hongjie Li

  3. Cotton Research Institute, Shanxi Agricultural University (Shanxi Academy of Agricultural Sciences), Yuncheng, 044000, China

    Feng Li

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Contributions

CX conceived the project; LQ performed the experiments; NL, HW, QZ, WW assisted in phenotyping and genotyping of the populations; XS performed inoculation of twenty-five Bgt isolates; FL analyzed the SNP data; WG analyzed the re-sequenced data; ZH, HL, JM, QS assisted in revising the manuscript; LQ and CX analyzed the experimental results and wrote the manuscript.

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Correspondence to Chaojie Xie.

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Qiu, L., Liu, N., Wang, H. et al. Fine mapping of a powdery mildew resistance gene MlIW39 derived from wild emmer wheat (Triticum turgidum ssp. dicoccoides). Theor Appl Genet 134, 2469–2479 (2021). https://doi.org/10.1007/s00122-021-03836-9

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