Abstract
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This paper reports fine mapping of qCLS for resistance to Cercospora leaf spot disease in mungbean and identified LOC106765332encoding TATA-binding-protein-associated factor 5 (TAF5) as the candidate gene for the resistance
Abstract
Cercospora leaf spot (CLS) caused by the fungus Cercospora canescens is an important disease of mungbean. A QTL mapping using mungbean F2 and BC1F1 populations developed from the “V4718” (resistant) and “Kamphaeng Saen 1” (KPS1; susceptible) has identified a major QTL controlling CLS resistance (qCLS). In this study, we finely mapped the qCLS and identified candidate genes at this locus. A BC8F2 [KPS1 × (KPS1 × V4718)] population developed in this study and the F2 (KPS1 × V4718) population used in a previous study were genotyped with 16 newly developed SSR markers. QTL analysis in the BC8F2 and F2 populations consistently showed that the qCLS was mapped to a genomic region of ~ 13 Kb on chromosome 6, which contains only one annotated gene, LOC106765332 (designated “VrTAF5”), encoding TATA-binding-protein-associated factor 5 (TAF5), a subunit of transcription initiation factor IID and Spt-Ada-Gcn5 acetyltransferase complexes. Sequence comparison of VrTAF5 between KPS1 and V4718 revealed many single nucleotide polymorphisms (SNPs) and inserts/deletions (InDels) in which eight SNPs presented in eight different exons, and an SNP (G4,932C) residing in exon 8 causes amino acid change (S250T) in V4718. An InDel marker was developed to detect a 24-bp InDel polymorphism in VrTAF5 between KPS1 and V4718. Analysis by RT-qPCR showed that expression levels of VrTAF5 in KPS1 and V4718 were not statistically different. These results indicated that mutation in VrTAF5 causing an amino acid change in the VrTAF5 protein is responsible for CLS resistance in V4718.
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Acknowledgements
We are thankful to the Joint Legume Research Center between Jiangsu Academy of Agriculture Sciences and Kasetsart University for molecular lab facilities.
Funding
This work was supported by the National Key Research and Development Program of China (Grant No. 2016YFE0203800), the China Agricultural Research System (CARS-08), and the Jiangsu Agriculture Industry Technology System (Grant No. JATS[2018]255).
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PS conceived and designed the study. CY conducted all of the experiments. JC and XY participated in marker, DNA sequencing, and gene expression analyses. CY, PS, and SC developed the populations and conducted phenotyping. XC and PS acquired funding. PS and CX supervised the study. CY, PS, and XC wrote the manuscript. PS edited and revised the manuscript. All authors reviewed the manuscript.
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Supplementary file 1 (PDF 310 kb)
Supplementary Table S1 PCR primers used in amplification of a genomic region covering LOC106765332. Annealing temperature for all primers is 55-60 °C
Supplementary file 2 (PDF 563 kb)
Supplementary Table S2 PCR primers designed for real-time PCR analysis of LOC106765332 and the internal reference genes. Annealing temperature for all primers is 55°C
Supplementary file 3 (PDF 409 kb)
Supplementary Table S3 Mungbean SSR primers covering the marker intervals for qCLS developed in this study. Annealing temperature for all primers is 55°C
Supplementary file 4 (PDF 88 kb)
Supplementary Fig. S1 Scheme showing procedure for the development of BC8F2 mungbean population used for QTL mapping of Cercospora leaf spot resistance
Supplementary file 5 (PDF 190 kb)
Supplementary Fig. S2 Full sequence alignment of VrTAF5 (LOC106765332) from KPS1, V4718, and VC1973A (reference sequence)
Supplementary file 6 (PDF 178 kb)
Supplementary Fig. S3 Sequence alignment of the coding sequence of VrTAF5 (LOC106765332) from KPS1, V4718, and VC1973A (reference sequence)
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Yundaeng, C., Somta, P., Chen, J. et al. Fine mapping of QTL conferring Cercospora leaf spot disease resistance in mungbean revealed TAF5 as candidate gene for the resistance. Theor Appl Genet 134, 701–714 (2021). https://doi.org/10.1007/s00122-020-03724-8
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DOI: https://doi.org/10.1007/s00122-020-03724-8